Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses
Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses
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DOI：
10.1016/j.jviromet.2008.12.016
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发表时间：
2009-05-01
影响因子：
3.1
通讯作者：
Kok, Tuckweng
中科院分区：
文献类型：
--
作者：
Oh, Sawyin;Selleck, Paul;Kok, Tuckweng
描述了抗甲型流感/鸡/越南/8/2004 （H5N1）病毒的四种IgG（1K）单克隆抗体（mab）。其中3种对来自进化枝1的H5N1毒株显示出中和活性。2和3使用逆转录病毒假型或活病毒微量中和试验。在伪型试验中，IC90中和滴度范围为160 - 51200，微量中和滴度范围为80 - 10240，MAb 1C1在两种试验中均表现出较强的中和活性。通过免疫组织化学染色，所有四种单克隆抗体对免疫原有特异性反应，通过免疫荧光对A/Hong Kong/483/1997 （H5N1）和A/Thailand/1(KAN-1)/2004 （H5N1）感染的MDCK细胞有特异性反应。ELISA法测定的单抗对H5N1血凝素（HA）具有特异性，对其他15种甲型流感亚型无交叉反应性。Western blot结果显示，只有单克隆抗体1C1和未中和的1F7与HA(1)发生反应。这些结果表明，单克隆抗体识别不同或重叠的表位，将是构建特定的快速护理点检测或治疗用途的有用试剂。爱思唯尔B.V.版权所有
Four IgG(1K) monoclonal antibodies (mAbs) against Influenza A/Chicken/Vietnam/8/2004 (H5N1) virus are described. Three of these showed neutralizing activities against H5N1 strains from clades 1. 2 and 3 using a retroviral pseudotype or live virus microneutralization assay. In the pseudotype assay, the IC90 neutralizing titre range was >1600-51,200, and with the microneutralization was 80 > 10,240, MAb 1C1 showed strong neutralizing activities in both assays. All four mAbs reacted specifically to the immunogen by immunohistochemical staining and to A/Hong Kong/483/1997 (H5N1) and A/Thailand/1(KAN-1)/2004 (H5N1)-infected MDCK cells by immunofluorescence. ELISA titrations of the mAbs showed specificity for H5N1 haemagglutinin (HA) and no cross-reactivity to 15 other Influenza A subtypes. Only mAbs 1C1 and the non-neutralizing 1F7 reacted with HA(1), the cleaved subunit of HA, by Western blot. These results suggest that the mAbs recognize distinct or overlapping epitopes and will be useful reagents for construction of specific rapid point-of-care assays or for therapeutic use. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.