SELECTIVE STIMULATION OF BIFIDOBACTERIA IN THE HUMAN COLON BY OLIGOFRUCTOSE AND INULIN
SELECTIVE STIMULATION OF BIFIDOBACTERIA IN THE HUMAN COLON BY OLIGOFRUCTOSE AND INULIN
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DOI：
10.1016/0016-5085(95)90192-2
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发表时间：
1995-04-01
期刊：
影响因子：
29.4
通讯作者：
CUMMINGS, JH
中科院分区：
文献类型：
--
作者：
GIBSON, GR;BEATTY, ER;CUMMINGS, JH
背景/目的：低聚果糖和菊粉是天然存在的难以消化的碳水化合物。在体外，它们选择性地刺激双歧杆菌的生长，双歧杆菌是一种被认为对健康有益的细菌。本研究旨在确定它们对体内大肠菌群和结肠功能的影响。方法：8名受试者参与了一项为期45天的研究，在此期间他们食用了受控制的饮食。中间15天，15g。日(-1)低聚果糖替代15g。一天(-1)蔗糖。其中四名受试者继续服用15g。天(-1)菊粉。测定排便习惯、排便时间、大便成分、呼气中H-2和CH4，以及结肠细菌的优势属。结果：低聚果糖和菊粉均显著增加了双歧杆菌的数量，分别从8.8至9.5log(10)g粪便(-1)和9.2至10.1log(10)g粪便(-1)，而类杆菌、梭状芽孢杆菌和梭菌在喂食低聚果糖时减少，革兰氏阳性球菌在喂食菊粉时减少。细菌总数没有变化。粪便湿、干物质、氮和能量排泄量随着两种底物的增加而增加，呼吸H-2也是如此。粪便短链脂肪酸和呼气CH4变化不大。结论：A-15克。日粮中添加低聚果糖或菊粉使双歧杆菌成为粪便中数量最多的属。因此，饮食上的微小变化可以改变结肠细菌的平衡，使其倾向于潜在的更健康的微生物区系。
Background/Aims: Oligofructose and inulin ave naturally occurring indigestible carbohydrates. In vitro they selectively stimulate the growth of species of Bifidobacterium, a genus of bacteria considered beneficial to health. This study was designed to determine their effects on the large bowel microflora and colonic function in vivo. Methods: Eight subjects participated in a 45-day study during which they ate controlled diets. For the middle 15 days, 15 g . day(-1) oligofructose was substituted for 15 g . day(-1) sucrose. Four of these subjects went on to a further period with 15 g . day(-1) inulin. Bowel habit, transit time, stool composition, breath H-2 and CH4, and the predominant genera of colonic bacteria were measured. Results: Both oligofructose and inulin significantly increased bifidobacteria from 8.8 to 9.5 log(10) g stool(-1) and 9.2 to 10.1 log(10) g stool(-1), respectively, whereas bacteroides, clostridia, and fusobacteria decreased when subjects were fed oligofructose, and gram-positive cocci decreased when subjects were fed inulin. Total bacterial counts were unchanged. Fecal wet and dry matter, nitrogen, and energy excretion increased with both substrates, as did breath H-2. Little change in fecal short-chain fatty acids and breath CH4 was observed. Conclusions: A 15-g . day(-1) dietary addition of oligofructose or inulin led to Bifidobacterium becoming the numerically predominant genus in feces. Thus, small changes in diet can alter the balance of colonic bacteria towards a potentially healthier microflora.