A Poly(Lactic Acid-Co-Caprolactone)-Collagen Hybrid for Tissue Engineering Applications
A Poly(Lactic Acid-Co-Caprolactone)-Collagen Hybrid for Tissue Engineering Applications
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DOI：
10.1089/ten.tea.2008.0194
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发表时间：
2009-07-01
影响因子：
4.1
通讯作者：
Mudera, Vivek
中科院分区：
文献类型：
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作者：
Ananta, M.;Aulin, Cecilia E.;Mudera, Vivek
由合成聚合物聚（乳酸-共-己内酯）（PLACL）和天然来源的聚合物胶原蛋白组成的可生物降解的混合支架通过将过度水合的胶原蛋白凝胶塑性压缩到扁平经编PLACL补片上来构建。对胶原蛋白压实过程进行了表征，发现持续时间（而不是在塑性压缩的测试条件下施加的载荷）是携带细胞的组分中胶原蛋白和细胞密度的决定因素。将细胞在空间上分布在三种不同的设置中，并静态培养7天。用AlamarBlue定量评估混合结构的短期生物相容性，并用荧光染色和共聚焦显微镜定性评估。在间质等同物的塑性压缩后没有观察到显著的细胞死亡，证实了先前关于良好的细胞活力保留的报道。间质、上皮和复合组织等同物显示没有收缩的肉眼可见迹象，细胞增殖良好，7天内细胞数量增加2 - 3倍。定量分析显示细胞分布均匀，生物相容性良好。结果表明，可以在几个小时的时间内使用PLACL-胶原蛋白杂交构建体工程化活的和增殖的多层组织等同物。
A biodegradable hybrid scaffold consisting of a synthetic polymer, poly(lactic acid-co-caprolactone) (PLACL), and a naturally derived polymer, collagen, was constructed by plastic compressing hyperhydrated collagen gels onto a flat warp-knitted PLACL mesh. The collagen compaction process was characterized, and it was found that the duration, rather than the applied load under the test conditions in the plastic compression, was the determining factor of the collagen and cell density in the cell-carrying component. Cells were spatially distributed in three different setups and statically cultured for a period of 7 days. Short-term biocompatibility of the hybrid construct was quantitatively assessed with AlamarBlue and qualitatively with fluorescence staining and confocal microscopy. No significant cell death was observed after the plastic compression of the interstitial equivalents, confirming previous reports of good cell viability retention. The interstitial, epithelial, and composite tissue equivalents showed no macroscopic signs of contraction and good cell proliferation with a two- to threefold increase in cell number over 7 days. Quantitative analysis showed a homogenous cell distribution and good biocompatibility. The results indicate that viable and proliferating multilayered tissue equivalents can be engineered using the PLACL-collagen hybrid construct in the space of several hours.