Enhanced pathway efficiency of Saccharomyces cerevisiae by introducing thermo-tolerant devices
Enhanced pathway efficiency of Saccharomyces cerevisiae by introducing thermo-tolerant devices
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通过引入耐热装置提高酿酒酵母的途径效率
DOI：
10.1016/j.biortech.2014.07.063
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发表时间：
2014-10-01
影响因子：
11.4
通讯作者：
Li, Chun
中科院分区：
文献类型：
--
作者：
Liu, Yueqin;Zhang, Genli;Li, Chun
本研究设计了由嗜热菌的热休克基因组成的耐热装置，并将其引入酿酒酵母中，以提高其耐热性。在10株耐高温工程酵母中，T.TE-TTE2469、T.TE-GroS2和T.TE-IbpA的细胞密度比对照提高了25%以上，细胞活力提高了1.5~4倍。耐热菌株的生理特性表明，耐热装置保存了较好的细胞壁完整性、较高的海藻糖含量和较高的代谢能量。以耐高温工程菌株为研究对象，通过引入β-支链淀粉合成途径，考察了耐热装置对途径效率的影响，结果表明，β-支原体滴度提高了28.1%，生长温度范围扩大了28-35℃，发酵周期缩短了72 h。结果表明，将嗜热菌的热休克蛋白注入酿酒酵母是提高其耐热性的有效途径。(C)2014爱思唯尔有限公司。保留所有权利。
In this study, thermo-tolerant devices consisting of heat shock genes from thermophiles were designed and introduced into Saccharomyces cerevisiae for improving its thermo-tolerance. Among ten engineered thermo-tolerant yeasts, T.te-TTE2469, T.te-GroS2 and T.te-IbpA displayed over 25% increased cell density and 1.5-4-fold cell viability compared with the control. Physiological characteristics of thermo-tolerant strains revealed that better cell wall integrity, higher trehalose content and enhanced metabolic energy were preserved by thermo-tolerant devices. Engineered thermo-tolerant strain was used to investigate the impact of thermo-tolerant device on pathway efficiency by introducing beta-amyrin synthesis pathway, showed 28.1% increased beta-amyrin titer, 28-35 degrees C broadened growth temperature range and 72 h shortened fermentation period. The results indicated that implanting heat shock proteins from thermophiles to S. cerevisiae would be an efficient approach to improve its thermo-tolerance. (C) 2014 Elsevier Ltd. All rights reserved.