Microbial diversity of Indian Ocean hydrothermal vent plumes:: Microbes tolerant of desiccation, peroxide exposure, and ultraviolet and γ-irradiation
Microbial diversity of Indian Ocean hydrothermal vent plumes:: Microbes tolerant of desiccation, peroxide exposure, and ultraviolet and γ-irradiation
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DOI：
10.1089/ast.2006.0060
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发表时间：
2007-04-01
期刊：
影响因子：
4.2
通讯作者：
Venkateswaran, Kasthuri
中科院分区：
文献类型：
--
作者：
La Duc, Myron T.;Benardini, James N.;Venkateswaran, Kasthuri
卡利烟囱羽流是印度洋罗德里格斯三重交界处热液喷口场（深度约 2,240 m）的一部分，对卡利烟囱羽流的微生物多样性进行了检查，试图发现对这种恶劣环境进化出独特抵抗能力的“极端耐受”微生物。从通风口和距烟囱及其周围不同距离（2-20 m）的沉积物中收集水和沉积物样品。对样本进行了筛选，寻找能够承受多种压力的超耐受微生物。总共选择了 46 个分离株进行一系列扰动，例如热休克、干燥、H2O2、紫外线 (UV) 和伽马射线照射。冷杆菌属的存活。 L0S3S-03b 暴露于 > 1,000 J/m(2) UV254 辐射后，在不同程度的抵抗力背景下尤其令人感兴趣。植物人。这种非孢子形成微生物的细胞不仅在所有处理中都存活下来，而且在暴露于模拟火星紫外线辐射时表现出 30 秒的 90% 致死剂量，在暴露于全光谱紫外线时表现出 2 分钟的 100% 致死剂量，这与细菌内生孢子的研究结果相当。
The microbial diversity of Kali chimney plumes, part of a hydrothermal vent field in the Rodriguez Triple Junction, Indian Ocean (depth approximately 2,240 m), was examined in an attempt to discover "extremotolerant" microorganisms that have evolved unique resistance capabilities to this harsh environment. Water and sediment samples were collected from the vent and from sediments located at various distances (2-20 m) away from and surrounding the chimney. Samples were screened for hypertolerant microbes that are able to withstand multiple stresses. A total of 46 isolates were selected for exposure to a number of perturbations, such as heat shock, desiccation, H2O2, and ultraviolet (UV) and gamma-irradiation. The survival of Psychrobacter sp. L0S3S-03b following exposure to > 1,000 J/m(2) UV254 radiation was particularly intriguing amid a background of varying levels of resistance. Vegetative. cells of this non-spore-forming microbe not only survived all of the treatments, but also exhibited a 90% lethal dose of 30 s when exposed to simulated martian UV radiation and a 100% lethal dose of 2 min when exposed to full spectrum UV, which is comparable to findings for bacterial endospores.