Aggravation of inflammatory bowel diseases by oral streptococci
Aggravation of inflammatory bowel diseases by oral streptococci
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DOI：
10.1111/odi.12125
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发表时间：
2014-05-01
期刊：
影响因子：
3.8
通讯作者：
Nakano, K.
中科院分区：
文献类型：
--
作者：
Kojima, A.;Nomura, R.;Nakano, K.
目的变形链球菌可加重小鼠结肠炎。我们评估了结肠炎的毒力，使用型菌株，以及血液分离的几个口腔链球菌species.Materials和MethodsWe调查的敏感性，血液分离的几个口腔链球菌吞噬，粘附和侵入肝细胞和干扰素-γ分泌。使用葡聚糖硫酸钠诱导的结肠炎的小鼠模型来评估结肠炎的细菌恶化。此外，干扰素-γ抗体给药的小鼠结肠炎的突出加重。ResultsIn体外分析表明，血链球菌ATCC 10556是一个可能的强毒株之间的几个口腔链球菌的模式菌株，和血链球菌TW 289的血液分离株的分析揭示了一个潜在的强毒株。ATCC 10556和TW 289静脉给药导致葡聚糖硫酸钠诱导的结肠炎显著加重，组织病理学检查显示，由于肝细胞感染导致的干扰素-γ分泌导致结肠炎加重。管理干扰素-γ抗体抑制TW 289-诱导colis.ConclusionThese结果表明，一些致命的口服链球菌菌株与结肠炎的加重诱导增强分泌干扰素-γ时，他们侵入血液。
ObjectivesStreptococcus mutans can aggravate colitis in mice. We evaluated the virulence of colitis using type strains as well as blood isolates of several oral streptococcal species.Materials and MethodsWe investigated the susceptibility of blood isolates of several oral streptococci to phagocytosis, adhesion to and invasion of hepatic cells and interferon-gamma secretion. A mouse model of dextran sodium sulphate-induced colitis was used to evaluate bacterial aggravation of colitis. In addition, interferon-gamma antibody was administered to mice with prominent aggravation of colitis.ResultsIn vitro analyses showed that Streptococcus sanguinis ATCC 10556 was a possible virulent strain among type strains of several oral streptococci, and that analysis of blood isolates of S.sanguinis TW289 revealed a potential virulent strain. Intravenous administration of ATCC 10556 and TW289 caused prominent aggravation of dextran sodium sulphate-induced colitis, and histopathological examinations showed that interferon-gamma secretion due to infection of hepatic cells caused colitis aggravation. Administration of interferon-gamma antibody suppressed TW289-induced colitis.ConclusionThese results suggest that some virulent oral streptococcal strains are associated with the aggravation of colitis induced by enhanced secretion of interferon-gamma when they invade the bloodstream.