Activation of GPER suppresses epithelial mesenchymal transition of triple negative breast cancer cells via NF-κB signals
Activation of GPER suppresses epithelial mesenchymal transition of triple negative breast cancer cells via NF-κB signals
复制标题
GPER 的激活通过 NF-κ B 信号抑制三阴性乳腺癌细胞的上皮间质转化
DOI：
10.1016/j.molonc.2016.01.002
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发表时间：
2016-06-01
影响因子：
6.6
通讯作者：
Wang, Hong-Sheng
中科院分区：
文献类型：
--
作者：
Chen, Zhuo-Jia;Wei, Wei;Wang, Hong-Sheng
The targeted therapy for triple-negative breast cancer (TNBC) is a great challenge due to our poor understanding on its molecular etiology. In the present study, our clinical data showed that the expression of G-protein coupled estrogen receptor (GPER) is negatively associated with lymph node metastasis, high-grade tumor and fibronectin (FN) expression while positively associated with the favorable outcome in 135 TNBC patients. In our experimental studies, both the in vitro migration and invasion of TNBC cells were inhibited by GPER specific agonist G-1, through the suppression of the epithelial mesenchymal transition (EMT). The G-1 treatment also reduced the phosphorylation, nuclear localization, and transcriptional activities of NF-kappa B. While over expression of NF-kappa B attenuated the action of G-1 in suppressing EMT. Our data further illustrated that the phosphorylation of GSK-3 beta by PI3K/Akt and ERK1/2 mediated, at least partially, the inhibitory effect of G-1 on NF-kappa B activities. It was further confirmed in a study of MDA-MB-231 tumor xenografts in nude mice. The data showed that G-1 inhibited the in vivo growth and invasive potential of TNBC via suppression of EMT. Our present study demonstrated that an activation of GPER pathway elicits tumor suppressive actions on TNBC, and supports the use of G-1 therapeutics for TNBC metastasis. (C) 2016 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
