Gene Expression Profiles by Microarray Analysis during Matrigel-induced Tube Formation in a Human Extravillous Trophoblast Cell line: Comparison with Endothelial Cells
Gene Expression Profiles by Microarray Analysis during Matrigel-induced Tube Formation in a Human Extravillous Trophoblast Cell line: Comparison with Endothelial Cells
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DOI：
10.1016/j.placenta.2008.07.015
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发表时间：
2008-10-01
期刊：
影响因子：
3.8
通讯作者：
Wake, N.
中科院分区：
文献类型：
--
作者：
Fukushima, K.;Murata, M.;Wake, N.
胎盘发生过程中绒毛外细胞滋养层细胞（EVT）的血管内分化诱导螺旋动脉重塑，增加了绒毛内空间的血流，是维持妊娠所必需的。为了阐明这种分化中涉及的分子机制，我们研究了在基质胶诱导的人永生化EVT细胞系TCL 1细胞和人脐血管内皮细胞HUV-EC-C中的管形成期间的基因表达谱，并比较了它们的谱。（在3和6小时均> 3倍表达，和/或在3或6小时> 5倍表达）（在3和6小时均为0.33倍表达，和/或在3或6小时均小于0.2倍表达）分别为969和659。在HUV-EC-C中，表现出显著上调和下调的基因数目分别为86和65。在1628个基因中，只有73个基因在TCL 1中显示出显著的表达变化，这些基因与HUV-EC-C共有。结果表明，在血管内皮细胞中，与血管内皮细胞的细胞代谢、增殖、抗凋亡、蛋白水解、粘附等相关的基因在TCL 1中表达显著变化，其中一些基因参与了血管内皮细胞的分化和血管生成，其表达谱与内皮细胞有很大的不同。根据目前的数据进行进一步的研究可能有助于阐明正常和异常胎盘发生的机制。(c)2008爱思唯尔有限公司保留所有权利。
Endovascular differentiation of extravillous cytotrophoblasts (EVT) during placentogenesis induces remodeling of spiral arteries that increases blood flow toward the intravillous space and is required for maintaining pregnancy. To address the molecular mechanisms involved in this differentiation, we investigated the gene expression profile during matrigel-induced tube formation in TCL1 cells, a human immortalized EVT cell line, and HUV-EC-C, human umbilical vessel endothelial cells, and compared their profiles.The numbers of genes that showed significant up-regulation (> 3-fold expression at both 3 and 6 h, and/or > 5-fold expression at either 3 or 6 h) during tube formation and significant down-regulation (0.33-fold expression at both 3 and 6 h, and/or less than 0.2-fold expression at either 3 or 6 h), were 969 and 659 in TCL1, respectively. In HUV-EC-C, the numbers of genes that showed significant up-regulation and down-regulation were 86 and 65, respectively. Only 73 of 1628 genes that showed significant expression changes in TCL1 were common with HUV-EC-C. The genes showing significant expression change specifically in TCL1 were associated with cellular, metabolisms, proliferation, anti-apoptosis, proteolysis adhesion, and some known to be involved in EVT differentiation or related to angiogenesis.The gene expression profile in EVT during tube formation is very different from that of endothelial cells. Further investigations based on the current data may help to elucidate mechanisms of normal and abnormal placentogenesis. (c) 2008 Elsevier Ltd. All rights reserved.