A soft lithographic approach to fabricate patterned microfluidic channels
A soft lithographic approach to fabricate patterned microfluidic channels
复制标题
DOI：
10.1021/ac035415s
复制
发表时间：
2004-07-01
影响因子：
7.4
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Khademhosseini, A;Suh, KY;Langer, R
微流体通道内功能分子的表面特性和空间呈现的控制对于诊断分析和微反应器的开发以及细胞生物学和流体力学的基础研究非常重要。在这里，我们提出了一种简单的技术，适用于许多软光刻方法，通过精确控制基板的空间特性来制造坚固的微通道。在这种方法中，通过控制聚（二甲基硅氧烷）（PDMS）印模的尺寸并在等离子体处理过程中将印模留在适当的位置，可以保护图案化区域免受氧等离子体的影响。然后移除 PDMS 印模，微流体模具不可逆地粘合到基底上。该方法用于在微流体通道内对非生物污染的聚（乙二醇）基共聚物或多糖透明质酸进行图案化。然后使用这些非生物污染图案来制造纤连蛋白和牛血清白蛋白以及哺乳动物细胞的阵列。此外，使用层流实现了对多种蛋白质在多个或单个图案上沉积的进一步控制。此外，在通道内形成图案的细胞仍然存活并能够进行细胞内反应，并且可能被裂解以进行分析。
The control of surface properties and spatial presentation of functional molecules within a microfluidic channel is important for the development of diagnostic assays and microreactors and for performing fundamental studies of cell biology and fluid mechanics. Here, we present a simple technique, applicable to many soft lithographic methods, to fabricate robust microchannels with precise control over the spatial properties of the substrate. In this approach, the patterned regions were protected from oxygen plasma by controlling the dimensions of the poly(dimethylsiloxane) (PDMS) stamp and by leaving the stamp in place during the plasma treatment process. The PDMS stamp was then removed, and the microfluidic mold was irreversibly bonded to the substrate. The approach was used to pattern a nonbiofouling poly(ethylene glycol)-based copolymer or the polysaccharide hyaluronic acid within microfluidic channels. These nonbiofouling patterns were then used to fabricate arrays of fibronectin and bovine serum albumin as well as mammalian cells. In addition, further control over the deposition of multiple proteins onto multiple or individual patterns was achieved using laminar flow. Also, cells that were patterned within channels remained viable and capable of performing intracellular reactions and could be potentially lysed for analysis.