Evaluation of larval growth process and bamboo consumption of the bamboo powder-post beetle Dinoderus minutus using X-ray computed tomography
Evaluation of larval growth process and bamboo consumption of the bamboo powder-post beetle Dinoderus minutus using X-ray computed tomography
复制标题
利用 X 射线计算机断层扫描评估竹粉甲虫 Dinoderus minutus 幼虫的生长过程和竹子消耗
DOI：
10.1007/s10086-014-1458-3
复制
发表时间：
2015
影响因子：
2.9
通讯作者：
Yoshihisa Fujii
中科院分区：
文献类型：
--
作者：
Hiroki Watanabe;Yoshiyuki Yanase;Yoshihisa Fujii
竹粉虫Dinoderus minututuus是日本砍伐竹子的主要害虫。在这项研究中，利用X射线计算机断层扫描(CT)来非破坏性地追踪幼虫的运动。并评估幼虫对竹子的食用量。感染的标本，已被成虫D.每隔2至5天使用微焦点X射线CT系统对微小甲虫进行扫描。在CT图像上可以清楚地识别幼虫和其他阶段的甲虫，体素大小为61.9xμm，并与竹子、昆虫隧道和草皮区分开来。对幼虫进行追踪，直到化蛹，并测量其隧道的长度和体积，以评估它们对竹子的消耗。在本实验条件下，在平均22天的观察期内，一头幼虫钻取的隧道长度和体积估计分别为0.98亿mm和1.06亿mm~3(0.70 mg烘干质量)/天，幼虫活动期为390d。
The bamboo powder-post beetleDinoderus minutusis a major pest of felled bamboo in Japan. In this study, X-ray computed tomography (CT) was utilized to non-destructively trace the movement of the larvae ofD. minutusinside infested bamboo specimens and to evaluate the bamboo consumption of the larvae. The infested specimens, which had been enclosed with adultD. minutusbeetles, were scanned using a microfocus X-ray CT system every 2 to 5 days. Larvae and other stages of the beetles were clearly recognizable in the CT images with a voxel size of 61.9 μm, and they were distinguishable from the bamboo, insect tunnels, and frass. The larvae were traced until pupation, and the length and volume of their tunnels were measured to evaluate their bamboo consumption. The tunnel length and volume bored by one larva was estimated to be 0.98 mm and 1.06 mm3(0.70 mg of oven-dry mass) per day within the average observation period of 22 days, respectively, and the active larval period was estimated to be 39 days in our experimental conditions.