Effect of a biofilm‐degrading enzyme from an oral pathogen in transgenic tobacco on the pathogenicity of Pectobacterium carotovorum subsp. carotovorum
Effect of a biofilm‐degrading enzyme from an oral pathogen in transgenic tobacco on the pathogenicity of Pectobacterium carotovorum subsp. carotovorum
复制标题
转基因烟草中口腔病原体的生物膜降解酶对胡萝卜食果杆菌亚种致病性的影响。
DOI：
--
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
N. Ramasubbu
中科院分区：
文献类型：
--
作者：
C. Ragunath;M. Shanmugam;M. Bendaoud;J. Kaplan;N. Ramasubbu
The hypothesis that dispersin B (DspB), an enzyme from the periodontal pathogen Aggregatibacter actinomycetemcomitans that degrades the extracellular matrix polysaccharide PGA, will inhibit biofilm formation of the soft rot pathogen Pectobacterium carotovorum subsp. carotovorum in infected plants was tested by constitutive expression of DspB in tobacco plants. All the transgenic plants expressed properly folded and active DspB enzyme, although at different expression levels. In virulence assays, even the transgenic plant line D10, which produced a low level of DspB compared to other lines, showed significant resistance against P. carotovorum subsp. carotovorum, suggesting that DspB could be a valuable agent for biological control of P. carotovorum subsp. carotovorum infection in crop plants.
