A novel water-soluble β-D-glucan isolated from the spores of Ganoderma lucidum
A novel water-soluble β-D-glucan isolated from the spores of Ganoderma lucidum
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DOI：
10.1016/j.carres.2012.02.029
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发表时间：
2012-05-15
影响因子：
3.1
通讯作者：
Ding, Kan
中科院分区：
文献类型：
--
作者：
Dong, Qun;Wang, Ying;Ding, Kan
灵芝是东亚地区广泛使用的食用和药用蘑菇。近年来，其孢子已被用作补充剂与其他形式的抗肿瘤疗法相结合。灵芝孢子细胞壁含有大量的多糖。本研究通过热水提取、分级乙醇沉淀、阴离子交换色谱和凝胶渗透色谱从灵芝破壁孢子中分离得到中性多糖GLSA50-1B。通过糖成分分析、甲基化分析、部分酸水解、丙酮解以及NMR和ESI-MS光谱，GLSA50-1B被阐明是一种新型β-D-葡聚糖，其特征是具有1,6-连接的β-D-Glcp主链，具有不同长度的支链，由末端和1,4-连接的Glcp残基组成，连接到主链中的O-4个替代Glc残基。 (C) 2012 Elsevier Ltd. 保留所有权利。
Ganoderma lucidum is an edible and medicinal mushroom used widely in East Asia. In recent years, its spores have been used as a supplement in combination with other forms of antitumor therapies. The cell wall of Ganoderma lucidum spores contains a high amount of polysaccharides. In this study, a neutral polysaccharide, GLSA50-1B, was isolated from sporoderm-broken spores of Ganoderma lucidum, by hot-water extraction, graded ethanol precipitation, anion-exchange chromatography, and gel permeation chromatography. Using sugar compositional analysis, methylation analysis, partial acid hydrolysis, acetolysis, and NMR and ESI-MS spectroscopy, GLSA50-1B was elucidated to be a novel beta-D-glucan featured by a 1,6-linked beta-D-Glcp backbone with different length of branches consisting of terminal and 1,4-linked Glcp residues, attached to O-4 of alternative Glc residues in the backbone. (C) 2012 Elsevier Ltd. All rights reserved.