Surface electrodes record and label brain neurons in insects
Surface electrodes record and label brain neurons in insects
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DOI：
10.1152/jn.00490.2017
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发表时间：
2017-11-01
影响因子：
2.5
通讯作者：
Hedwig, Berthold
中科院分区：
文献类型：
--
作者：
Kostarakos, Konstantinos;Hedwig, Berthold
我们使用抽吸电极来可靠地记录蟋蟀大脑前表面识别的上行听觉中间神经元的活动。电极轻轻地附着在覆盖着前脑内上行中间神经元和环状听神经元投射区的鞘上。记录的特异性和选择性由精确的电极位置决定，电极位置可以很容易地改变而不会对组织造成损伤。在大脑表面的其他位置记录了不同的非听觉纤维;稳定的记录持续了几个小时。同样的电极被用于通过电泳将荧光示踪剂递送到神经系统中。这使我们能够逆行标记记录的听觉神经元，并揭示它们在第一胸神经节中的细胞体和树突结构。通过调整注入的染料量，我们对大脑中的环状听觉神经元进行了特异性染色，展示了对它有贡献的细胞体簇。我们的数据证明了表面电极是分析无脊椎动物小大脑中神经处理的通用工具。新&值得注意的是，我们表明表面抽吸电极可用于监测蟋蟀大脑中听觉神经元的活动。它们还允许以荧光示踪剂的方式递送，以标记记录的神经元和电极所连接的局部神经元的结构。这种新的细胞外记录和标记技术是一种多功能和有用的方法，探索在无脊椎动物的感觉和运动系统的神经加工。
We used suction electrodes to reliably record the activity of identified ascending auditory interneurons from the anterior surface of the brain in crickets. Electrodes were gently attached to the sheath covering the projection area of the ascending interneurons and the ringlike auditory neuropil in the protocerebrum. The specificity and selectivity of the recordings were determined by the precise electrode location, which could easily be changed without causing damage to the tissue. Different nonauditory fibers were recorded at other spots of the brain surface; stable recordings lasted for several hours. The same electrodes were used to deliver fluorescent tracers into the nervous system by means of electrophoresis. This allowed us to retrograde label the recorded auditory neurons and to reveal their cell body and dendritic structure in the first thoracic ganglion. By adjusting the amount of dye injected, we specifically stained the ringlike auditory neuropil in the brain, demonstrating the clusters of cell bodies contributing to it. Our data provide a proof that surface electrodes are a versatile tool to analyze neural processing in small brains of invertebrates.NEW & NOTEWORTHY We show that surface suction electrodes can be used to monitor the activity of auditory neurons in the cricket brain. They also allow delivering electrophoretically a fluorescent tracer to label the structure of the recorded neurons and the local neuropil to which the electrode was attached. This new extracellular recording and labeling technique is a versatile and useful method to explore neural processing in invertebrate sensory and motor systems.