Aging extension and modifications of lipid metabolism in the monogonont rotifer Brachionus koreanus under chronic caloric restriction.
Aging extension and modifications of lipid metabolism in the monogonont rotifer Brachionus koreanus under chronic caloric restriction.
复制标题
DOI：
10.1038/s41598-018-20108-7
复制
发表时间：
2018-01-29
影响因子：
4.6
通讯作者：
Lee JS
中科院分区：
文献类型：
--
作者：
Lee MC;Park JC;Yoon DS;Han J;Kang S;Kamizono S;Om AS;Shin KH;Hagiwara A;Lee JS
为了研究在慢性热量限制（CCR;浓度降低的绿色藻四藻suecica）的单角体轮虫朝鲜臂尾轮虫的衰老延长和脂质代谢的修改的相互关系，我们评估了生命周期参数，脂肪酸组成，和表达sirtuin和脂质代谢相关的基因。B。koreanus在5% T. Suecica组寿命延长，但繁殖能力下降。基于这一发现，我们选择了5% T。并与100%T.苏西卡在CCR下观察到沉默调节蛋白基因表达的上调。此外，尽管总脂肪酸（FA）的量和三酰甘油的面积减少，饱和脂肪酸和单不饱和脂肪酸（MUFA）的比例增加，总FA在5%暴露的B。koreanus进行观察。mRNA表达分析证实CCR通过Δ9去饱和酶促进MUFA的合成。此外，二十二碳六烯酸（DHA）合成基因Δ4去饱和酶的表达也上调，以及DHA含量。这些数据表明，CCR改变蛋白质乙酰化和脂质代谢，导致生殖减少，从而导致寿命延长。
To examine the interrelationship of aging extension and modification of lipid metabolism under chronic caloric restriction (CCR; reduced concentration of the green algae Tetraselmis suecica) in the monogonont rotifer Brachionus koreanus, we assessed life cycle parameters, fatty acid composition, and expression of sirtuin and genes related to lipid metabolism. B. koreanus in the 5% T. suecica group showed an increased life span but decreased reproduction. Based on this finding, we chose 5% T. suecica for further experiments and compared the data with those for 100% T. suecica. Upregulation of sirtuin gene expression was observed under CCR. In addition, despite the reduction in the amount of total fatty acid (FA) and the area of triacylglycerol, increases in the ratios of saturated fatty acid and monounsaturated fatty acid (MUFA) to total FA in 5%-exposed B. koreanus were observed. Furthermore, mRNA expression analysis confirmed that CCR promoted the synthesis of MUFA through Δ9 desaturase. Moreover, expression of the docosahexaenoic acid (DHA) synthesizing gene Δ4 desaturase was also upregulated, together with DHA content. These data suggest that CCR modified protein acetylation and lipid metabolism, leading to a decrease in reproduction and consequently resulting in life span extension.
登录
查看更多内容
影响因子：
3.9
作者：
Johnston RK;Snell TW
通讯作者：
Snell TW
影响因子：
64.8
作者：
Burnett, Camilla;Valentini, Sara;Cabreiro, Filipe;Goss, Martin;Somogyvari, Milan;Piper, Matthew D.;Hoddinott, Matthew;Sutphin, George L.;Leko, Vid;McElwee, Joshua J.;Vazquez-Manrique, Rafael P.;Orfila, Anne-Marie;Ackerman, Daniel;Au, Catherine;Vinti, Giovanna;Riesen, Michele;Howard, Ken;Neri, Christian;Bedalov, Antonio;Kaeberlein, Matt;Soti, Csaba;Partridge, Linda;Gems, David
通讯作者：
Gems, David
影响因子：
1.3
作者：
Kauler, Paul;Enesco, Hildegard E.
通讯作者：
Enesco, Hildegard E.
影响因子：
2.6
作者：
Sarma, SSS;Nandini, S;Gulati, RD
通讯作者：
Gulati, RD
DOI：
10.1093/gerona/glt160
发表时间：
2014-08-01
影响因子：
5.1
作者：
Kuhla, Angela;Hahn, Steffi;Vollmar, Brigitte
通讯作者：
Vollmar, Brigitte