Interleukin 7 receptor activates PI3K/Akt/mTOR signaling pathway via downregulation of Beclin-1 in lung cancer
Interleukin 7 receptor activates PI3K/Akt/mTOR signaling pathway via downregulation of Beclin-1 in lung cancer
复制标题
肺癌中白细胞介素 7 受体通过下调 Beclin-1 激活 PI3K/Akt/mTOR 信号通路
DOI：
10.1002/mc.22933
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发表时间：
2019-03-01
影响因子：
4.6
通讯作者：
Jiang Guocheng
中科院分区：
文献类型：
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作者：
Ming Jian;Zhu Yunjia;Jiang Guocheng
白细胞介素-7（IL-7）可调节细胞增殖和凋亡，也可调节肿瘤淋巴管生成，但其是否调节肿瘤细胞的自噬尚不清楚。本研究旨在探讨IL-7与自噬相关标志物Beclin 1和mTOR的关系以及IL-7调控人肺癌细胞自噬的机制。检测Beclin 1和mTOR在肺癌细胞中的表达及其对肺癌患者预后的影响。Western blot和RT-PCR检测结果表明，IL-7通过下调Beclin 1的表达，激活PI3 K/Akt/mTOR信号通路。此外，Beclin 1和mTOR的表达与非小细胞肺癌（NSCLC）患者的临床分期和生存期密切相关。IL-7R、mTOR和肿瘤分期是肺癌的独立预测因子。综上所述，我们的研究结果为IL-7通过Beclin 1激活PI3 K/Akt/mTOR信号通路调节肺癌细胞自噬提供了证据。
Interleukin-7(IL-7) can regulate proliferation and apoptosis of cell and also regulate tumor lymphangiogenesis, but whether it regulating autophagy of tumor cells is not well known. We study the relationship between IL-7 and some autophagy-related markers, Beclin 1 and mammalian target of rapamycin (mTOR) and the mechanism of IL-7 in regulating autophagy of human lung cancer cells. We detected expression of Beclin 1 and mTOR in lung cancer cells and their impact on the prognosis of lung cancer patients. Using Western blot and Reverse Transcription PCR, we found that IL-7 activates PI3 K/Akt/mTOR signaling pathway by downregulated the expression of Beclin 1 in lung cancer cell lines. In addition, the expressions of Beclin 1 and mTOR were well correlated with clinical stages and survival of human non-small cell lung cancer (NSCLC) patients. IL-7R, mTOR, and tumor stage were the independent prognosticators in lung cancer. Taken together, our results provided evidence that IL-7 activates PI3 K/Akt/mTOR signaling pathway via Beclin 1 to regulate autophagy in lung cancer cells.