Three-dimensional average-shape atlas of the honeybee brain and its applications
Three-dimensional average-shape atlas of the honeybee brain and its applications
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DOI：
10.1002/cne.20644
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发表时间：
2005-11-07
影响因子：
2.5
通讯作者：
Menzel, R
中科院分区：
文献类型：
--
作者：
Brandt, R;Rohlfing, T;Menzel, R
神经网络的解剖基础通常由神经元的重建组成，这些神经元在不同的制备方法中被染色。神经元之间结构关系的现实模型需要一个共同的框架。在这里，我们提出了连接触角叶(AL)、蘑菇体(MB)和侧角的单投影神经元(PN)、内在蘑菇体神经元群(5型Kenyon细胞)和单个蘑菇体外部神经元(PE1)的三维重建，旨在组成蜜蜂嗅觉通路的组成部分。为了做到这一点，我们构建了蜜蜂大脑的数字标准图谱。标准图谱是由22个神经毛刺组成的平均形状图谱，这些图谱是从20个免疫染色的整体蜂脑计算得出的。在通过重复应用基于强度的非刚性配准算法来校正全局大小和定位差异之后，创建了平均标签图像序列。通过生成与平均标签图像相对应的平均灰度值图像并判断标签区域内的细节级别来对结果进行定性评估。我们发现，序列中的第一个仿射配准步骤会导致图像模糊，因为存在相当大的局部形状差异。然而，序列中的第一次非刚性迭代已经修正了个体之间的大部分形状差异，导致图像具有丰富的内部细节。第二次迭代在此基础上有所改进，并被选为标准。将不同制备的神经元登记到标准图谱中显示：1)m-ACT神经元占据整个肾小球(皮质和核心)并与皮质层的局部中间神经元重叠；2)在MB花瓣和原大脑叶的侧角，两个已鉴定的m-ACT神经元的轴突终末树枝状分布在不同的神经纤维紧密相连的区域；3)MB固有的爪状Kenyon细胞(5型)，胞体位于帽杯外，投射到MB的花梗和叶输出系统，并与垂直叶底部的PE1神经元的树突树相连(邻近)。因此，标准图谱和注册程序具有创建神经网络各部分的真实神经解剖学模型的功能。蜜蜂标准大脑的网址是www.urobiologie.fu-berlin.de/beeBrain。
The anatomical substrates of neural nets are usually composed from reconstructions of neurons that were stained in different preparations. Realistic models of the structural relationships between neurons require a common framework. Here we present 3-D reconstructions of single projection neurons (PN) connecting the antennal lobe (AL) with the mushroom body (MB) and lateral horn, groups of intrinsic mushroom body neurons (type 5 Kenyon cells), and a single mushroom body extrinsic neuron (PE1), aiming to compose components of the olfactory pathway in the honeybee. To do so, we constructed a digital standard atlas of the bee brain. The standard atlas was created as an average-shape atlas of 22 neuropils, calculated from 20 individual immunostained whole-mount bee brains. After correction for global size and positioning differences by repeatedly applying an intensity-based nonrigid registration algorithm, a sequence of average label images was created. The results were qualitatively evaluated by generating average gray-value images corresponding to the average label images and judging the level of detail within the labeled regions. We found that the first affine registration step in the sequence results in a blurred image because of considerable local shape differences. However, already the first nonrigid iteration in the sequence corrected for most of the shape differences among individuals, resulting in images rich in internal detail. A second iteration improved on that somewhat and was selected as the standard. Registering neurons from different preparations into the standard atlas reveals 1) that the m-ACT neuron occupies the entire glomerulus (cortex and core) and overlaps with a local interneuron in the cortical layer; 2) that, in the MB calyces and the lateral horn of the protocerebral lobe, the axon terminals of two identified m-ACT neurons arborize in separate but close areas of the neuropil; and 3) that MB-intrinsic clawed Kenyon cells (type 5), with somata outside the calycal cups, project to the peduncle and lobe output system of the MB and contact (proximate) the dendritic tree of the PE1 neuron at the base of the vertical lobe. Thus the standard atlas and the procedures applied for registration serve the function of creating realistic neuroanatomical models of parts of a neural net. The Honeybee Standard Brain is accessible at www.neurobiologie.fu-berlin.de/beebrain.