PROTEIN-SYNTHESIS IN SEMLIKI FOREST VIRUS-INFECTED CELLS IS NOT CONTROLLED BY PERMEABILITY CHANGES
PROTEIN-SYNTHESIS IN SEMLIKI FOREST VIRUS-INFECTED CELLS IS NOT CONTROLLED BY PERMEABILITY CHANGES
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DOI：
10.1099/0022-1317-64-12-2631
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发表时间：
1983-01-01
影响因子：
3.8
通讯作者：
PASTERNAK, CA
中科院分区：
文献类型：
--
作者：
GRAY, MA;AUSTIN, SA;PASTERNAK, CA
GTP类似物胍基（. β,. γ）的摄取。-亚甲基二膦酸盐（GppCH2p）在塞姆利基森林病毒（SFV）感染的[仓鼠肾]BHK细胞中与模拟感染的细胞中是相同的，尽管在SFV感染的细胞中，GppCH2p对蛋白质合成的抑制作用比对照细胞更明显。对这种差异的一种可能解释是，与未感染的细胞相比，感染细胞的GTP浓度较低，GTP:GDP的比例也较低，因此类似物可能是一种更有效的竞争性翻译抑制剂。在这个系统中，感染细胞和未感染细胞的区别不在于质膜，而在于细胞质。
The uptake of the GTP analog guanylyl(.beta.,.gamma.-methylene)diphosphonate (GppCH2p) is the same in Semliki Forest virus (SFV)-infected [hamster kidney] BHK cells as in mock-infected cells, in spite of the fact that protein synthesis is inhibited by GppCH2p more markedly in SFV-infected cells than in control cells. A possible explanation for this difference is that infected cells have a lower concentration of GTP and a lower ratio of GTP:GDP than uninfected cells, and the analog may thus be a more effective competitive inhibitor of translation. In this system, the difference between infected and uninfected cells lies not at the plasma membrane but within the cytoplasm.