Nuclear localization of eukaryotic class II release factor (eRF3): Implication for the multifunction of eRF3 in ciliates Euplotes cell
Nuclear localization of eukaryotic class II release factor (eRF3): Implication for the multifunction of eRF3 in ciliates Euplotes cell
复制标题
真核 II 类释放因子 (eRF3) 的核定位：对纤毛虫游标细胞中 eRF3 多功能的影响
DOI：
10.1016/j.cellbi.2007.12.005
复制
发表时间：
2008-03-01
影响因子：
3.9
通讯作者：
Liang, Aihua
中科院分区：
文献类型：
--
作者：
Chai, Baofeng;Wang, Wei;Liang, Aihua
II类多肽释放因子（Class II polypeptide release factor，eRF 3）是一种核糖体和eRF 1依赖的GT3，在蛋白质合成终止过程中与eRF 1协同作用，促进连接多肽链和肽基位点tRNA的酯键水解。我们制备了针对游仆虫eRF3的抗体，并通过免疫电镜在纤毛虫中进行了定位研究。我们的研究结果表明，eRF3是存在于细胞质和两种类型的细胞核的这种生物体。利用RNA干扰技术分析eRF3在这些细胞核中的功能。eRF 3基因干扰的游仆虫细胞核松散的形状，表明eRF 3可能参与细胞核的形态组织。这表明eRF3是一种多功能蛋白质，在蛋白质合成终止过程中具有除其功能外的作用。(C)2008年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
Class II polypeptide release factor (eRF3), a ribosome and eRF1-dependent GTPase, is an important factor, which acts cooperatively with eRF1 to promote hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA in process of termination of protein synthesis. We prepared antibodies against eRF3 of Euplotes octocarinatus, and performed localization studies by immunoelectron microscopy in the ciliate. Our results indicate that eRF3 is present both in the cytoplasm and the two types of nuclei of this organism. The functions of eRF3 in these nuclei were analyzed by RNA interference methods. The nuclei loose their shape in eRF3 gene-interfered Euplotes cells, suggesting that eRF3 is probably involved in the morphological organization of nuclei. This suggests that eRF3 is a multifunctional protein with roles additionals to its function in the process of termination of protein synthesis. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.