Impact of passing mesenchymal stem cells through smaller bore size needles for subsequent use in patients for clinical or cosmetic indications.
Impact of passing mesenchymal stem cells through smaller bore size needles for subsequent use in patients for clinical or cosmetic indications.
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DOI：
10.1186/1479-5876-10-229
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发表时间：
2012-11-21
影响因子：
7.4
通讯作者：
Das AK
中科院分区：
文献类型：
--
作者：
Mamidi MK;Singh G;Husin JM;Nathan KG;Sasidharan G;Zakaria Z;Bhonde R;Majumdar AS;Das AK
许多临床前和临床研究已经研究了间充质干细胞（MSCs）注射到靶器官后的再生潜力和营养支持。临床医生倾向于使用孔径最小的针头将msc输送到心脏、肝脏和脾脏等血管器官。人们一直担心，针头孔径小可能会损害这些细胞的健康，降低间充质干细胞的存活和可塑性。在本报告中，我们的目的是研究尽可能小孔径的针头，以支持MSCs安全输送到不同的临床或美容应用的各种组织中。为了完成这个实验，我们在实验室用24g、25g和26g的针头连接到1ml注射器上注射细胞，并无菌收集细胞。对照细胞不使用任何针头，通过1 ml注射器排出。随后，对针刺细胞进行培养，并对其形态、附着、活力、表型表达、分化潜力、冷冻保存和体内迁移能力进行了表征。在第二阶段的研究中，细胞通过26 G针头连接在1 ml注射器上注射10次。与对照组相比，抽吸组细胞表型和功能特征相似。单次和多次注射后，MSCs均保持其细胞和功能特性。本研究证明26g孔径的针头可以安全地用于临床/治疗目的注射MSCs。
Numerous preclinical and clinical studies have investigated the regenerative potential and the trophic support of mesenchymal stem cells (MSCs) following their injection into a target organ. Clinicians favor the use of smallest bore needles possible for delivering MSCs into vascular organs like heart, liver and spleen. There has been a concern that small needle bore sizes may be detrimental to the health of these cells and reduce the survival and plasticity of MSCs. In this report, we aimed to investigate the smallest possible bore size needle which would support the safe delivery of MSCs into various tissues for different clinical or cosmetic applications. To accomplish this we injected cells via needle sizes 24, 25 and 26 G attached to 1 ml syringe in the laboratory and collected the cells aseptically. Control cells were ejected via 1 ml syringe without any needle. Thereafter, the needle ejected cells were cultured and characterized for their morphology, attachment, viability, phenotypic expression, differentiation potential, cryopreservation and in vivo migration abilities. In the second phase of the study, cells were injected via 26 G needle attached to 1 ml syringe for 10 times. Similar phenotypic and functional characteristics were observed between ejected and control group of cells. MSCs maintained their cellular and functional properties after single and multiple injections. This study proves that 26 G bore size needles can be safely used to inject MSCs for clinical/therapeutics purposes.