Directing cell motions on micropatterned ratchets
Directing cell motions on micropatterned ratchets
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DOI：
10.1038/nphys1306
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发表时间：
2009-08-01
期刊：
影响因子：
19.6
通讯作者：
Grzybowski, Bartosz A.
中科院分区：
文献类型：
--
作者：
Mahmud, Goher;Campbell, Christopher J.;Grzybowski, Bartosz A.
细胞运动是一个源自细胞骨架同步动力学的过程。在几个重要的生理过程中，特别是癌症转移，随机运动的细胞可以获得定向运动表型，并根据环境线索偏向它们的运动。然而，尽管进行了大量的研究，但目前对定向细胞迁移的理解是不完整的，并且越来越需要开发能够研究和控制该过程的系统。这篇文章表明，运动细胞的随机运动可以纠正不对称（“棘轮”）的微观几何形状。细胞之间的相互作用和强加的几何线索引导细胞极化，并产生定向运动。根据棘轮设计，不同类型的细胞可以在相同的强加模式上沿相同或相反的方向移动。在后一种情况下，可以将混合的细胞群体部分分选到不同的收集库中。
Cell motility is a process deriving from the synchronized dynamics of the cytoskeleton. In several important physiological processes-notably, cancer metastasis-the randomly moving cells can acquire a directional motility phenotype and bias their motions in response to environmental cues. Despite intense research, however, the current understanding of directional cell migration is incomplete and there is a growing need to develop systems that would enable the study and control of this process. This article demonstrates that random motions of motile cells can be rectified by asymmetric ('ratchet') microgeometries. Interactions between the cells and the imposed geometrical cues guide cell polarization and give rise to directional motility. Depending on the ratchet design, cells of different types can move either in the same or in opposite directions on the same imposed pattern. In the latter case, it is possible to partially sort mixed cell populations into different collecting reservoirs.