Characterization of single nucleotide polymorphisms for a forward genetics approach using genetic crosses in C57BL/6 and BALB/c substrains of mice.
Characterization of single nucleotide polymorphisms for a forward genetics approach using genetic crosses in C57BL/6 and BALB/c substrains of mice.
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使用C57BL/6和小鼠BALB/C基质中的遗传杂交的单核苷酸多态性的表征。
DOI：
10.1538/expanim.21-0181
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发表时间：
2022-05-20
影响因子：
2.4
通讯作者：
Wakana, Shigeharu
中科院分区：
文献类型：
--
作者：
Miura, Ikuo;Kikkawa, Yoshiaki;Yasuda, Shumpei P.;Shinogi, Akiko;Usuda, Daiki;Kumar, Vivek;Takahashi, Joseph S.;Tamura, Masaru;Masuya, Hiroshi;Wakana, Shigeharu
正向遗传学是一种基于表型染色体作图的有效方法，并已成功地导致了许多小鼠与各种表型有关的基因突变的发现。虽然遗传距离较远的品系之间的杂交可以产生用于染色体定位的F2和回交小鼠，但表型经常受到遗传杂交中配对菌株的背景效应的影响。亚种间的遗传杂交在遗传作图中可能是有用的，以避免遗传背景效应。在这项研究中，我们利用C57BL/6和BALB/c小鼠的亚系，研究了可用于遗传图谱的单核苷酸多态(SNPs)。在C57BL/6小鼠中，开发了114个SNP标记，并将其分配到所有染色体上的位置，以便充分利用C57BL/6J和C57BL/6N亚株之间的遗传杂交进行遗传作图。此外，来自5个生产育种者的7个C57BL/6亚株在114个SNP标记上也存在遗传差异。此外，在BALB/cAJCL和BALB/cByJJCL亚株的所有染色体上均检测到106个SNPs。这些SNP可用于BALB/CJ、BALB/cAJCL、BALB/cAnNCrlCrlj和BALB/cCrSlc小鼠的基因分型，特别适用于利用BALB/cByJJc1和其他BALB/c亚株的杂交进行基因定位。本研究所鉴定的SNPs可用于遗传作图，以确定N-乙基-N-亚硝脲诱变引起的表型突变以及导致C57BL/6和BALB/c小鼠亚系表型差异的SNPs。
Forward genetics is a powerful approach based on chromosomal mapping of phenotypes and has successfully led to the discovery of many mouse mutations in genes responsible for various phenotypes. Although crossing between genetically remote strains can produce F2 and backcross mice for chromosomal mapping, the phenotypes are often affected by background effects from the partner strains in genetic crosses. Genetic crosses between substrains might be useful in genetic mapping to avoid genetic background effects. In this study, we investigated single nucleotide polymorphisms (SNPs) available for genetic mapping using substrains of C57BL/6 and BALB/c mice. In C57BL/6 mice, 114 SNP markers were developed and assigned to locations on all chromosomes for full utilization for genetic mapping using genetic crosses between the C57BL/6J and C57BL/6N substrains. Moreover, genetic differences were identified in the 114 SNP markers among the seven C57BL/6 substrains from five production breeders. In addition, 106 SNPs were detected on all chromosomes of BALB/cAJcl and BALB/cByJJcl substrains. These SNPs could be used for genotyping in BALB/cJ, BALB/cAJcl, BALB/cAnNCrlCrlj, and BALB/cCrSlc mice, and they are particularly useful for genetic mapping using crosses between BALB/cByJJcl and other BALB/c substrains. The SNPs characterized in this study can be utilized for genetic mapping to identify the causative mutations of the phenotypes induced by N-ethyl-N-nitrosourea mutagenesis and the SNPs responsible for phenotypic differences between the substrains of C57BL/6 and BALB/c mice.
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影响因子：
3.5
作者：
Masuya, H;Shimizu, K;Shiroishi, T
通讯作者：
Shiroishi, T
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
5.8
作者：
Li, Heng
通讯作者：
Li, Heng
影响因子：
64.8
作者：
Dickinson ME;Flenniken AM;Ji X;Teboul L;Wong MD;White JK;Meehan TF;Weninger WJ;Westerberg H;Adissu H;Baker CN;Bower L;Brown JM;Caddle LB;Chiani F;Clary D;Cleak J;Daly MJ;Denegre JM;Doe B;Dolan ME;Edie SM;Fuchs H;Gailus-Durner V;Galli A;Gambadoro A;Gallegos J;Guo S;Horner NR;Hsu CW;Johnson SJ;Kalaga S;Keith LC;Lanoue L;Lawson TN;Lek M;Mark M;Marschall S;Mason J;McElwee ML;Newbigging S;Nutter LM;Peterson KA;Ramirez-Solis R;Rowland DJ;Ryder E;Samocha KE;Seavitt JR;Selloum M;Szoke-Kovacs Z;Tamura M;Trainor AG;Tudose I;Wakana S;Warren J;Wendling O;West DB;Wong L;Yoshiki A;International Mouse Phenotyping Consortium;Jackson Laboratory;Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris (ICS);Charles River Laboratories;MRC Harwell;Toronto Centre for Phenogenomics;Wellcome Trust Sanger Institute;RIKEN BioResource Center;MacArthur DG;Tocchini-Valentini GP;Gao X;Flicek P;Bradley A;Skarnes WC;Justice MJ;Parkinson HE;Moore M;Wells S;Braun RE;Svenson KL;de Angelis MH;Herault Y;Mohun T;Mallon AM;Henkelman RM;Brown SD;Adams DJ;Lloyd KC;McKerlie C;Beaudet AL;Bućan M;Murray SA
通讯作者：
Murray SA
影响因子：
64.8
作者：
Funato H;Miyoshi C;Fujiyama T;Kanda T;Sato M;Wang Z;Ma J;Nakane S;Tomita J;Ikkyu A;Kakizaki M;Hotta-Hirashima N;Kanno S;Komiya H;Asano F;Honda T;Kim SJ;Harano K;Muramoto H;Yonezawa T;Mizuno S;Miyazaki S;Connor L;Kumar V;Miura I;Suzuki T;Watanabe A;Abe M;Sugiyama F;Takahashi S;Sakimura K;Hayashi Y;Liu Q;Kume K;Wakana S;Takahashi JS;Yanagisawa M
通讯作者：
Yanagisawa M