Identification and expression pattern of the chemosensory protein gene family in the silkworm, Bombyx mori
Identification and expression pattern of the chemosensory protein gene family in the silkworm, Bombyx mori
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家蚕化学感应蛋白基因家族的鉴定及表达模式
DOI：
10.1016/j.ibmb.2006.11.012
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发表时间：
2007-03-01
影响因子：
3.8
通讯作者：
Xiang, Zhong-Huai
中科院分区：
文献类型：
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作者：
Gong, Da-Ping;Zhang, Hui-jie;Xiang, Zhong-Huai
昆虫化学感觉蛋白（CSPs）和气味结合蛋白（OBPs）被认为是将疏水化学物质运输到感觉神经元上的受体。与OBPs相比，CSPs在各种昆虫组织中表达更广泛。我们对家蚕候选CSP基因家族进行了全基因组分析。根据其保守的半胱氨酸残基和与其他昆虫CSPs的相似性，共鉴定了20个候选CSPs，包括3个基因片段和2个假基因。其中一些基因聚集在蚕的基因组中。利用RTPCR和芯片技术分析了这些候选基因的表达谱，结果表明这些基因主要在成熟幼虫和成蛾中表达，提示家蚕CSPs可能参与了发育。家蚕CSP基因在触角、头、胸、腿、翅膀、上皮、睾丸、卵巢、信息素腺、翅盘、复眼等组织中广泛表达。(c) 2006 Elsevier Ltd.版权所有。
Insect chemosensory proteins (CSPs) as well as odorant-binding proteins (OBPs) have been supposed to transport hydrophobic chemicals to receptors on sensory neurons. Compared with OBPs, CSPs are expressed more broadly in various insect tissues. We performed a genome-wide analysis of the candidate CSP gene family in the silkworm. A total of 20 candidate CSPs, including 3 gene fragments and 2 pseudogenes, were characterized based on their conserved cysteine residues and their similarity to CSPs in other insects. Some of these genes were clustered in the silkworm genome. The gene expression pattern of these candidates was investigated using RTPCR and microarray, and the results showed that these genes were expressed primarily in mature larvae and the adult moth, suggesting silkworm CSPs may be involved in development. The majority of silkworm CSP genes are expressed broadly in tissues including the antennae, head, thorax, legs, wings, epithelium, testes, ovaries, pheromone glands, wing disks, and compound eyes. (c) 2006 Elsevier Ltd. All rights reserved.