Assessing bioenergetic functions from isolated mitochondria in Drosophila melanogaster.
Assessing bioenergetic functions from isolated mitochondria in Drosophila melanogaster.
复制标题
DOI：
10.14440/jbm.2016.112
复制
发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Ballard, J William O
中科院分区：
文献类型：
--
作者：
Aw, Wen C;Bajracharya, Rijan;Ballard, J William O
线粒体参与产生超过90%的细胞能量，并负责许多细胞过程，如代谢，细胞信号传导，凋亡和衰老。目前，有许多不同的实验方法用于测量线粒体的健康和功能。在这里，我们展示了一种新的方法，定量底物诱导的线粒体呼吸果蝇。该方案是优化的线粒体分离自三龄幼虫，也可以用于线粒体分离自成人胸部。该程序概述了如何对状态II、状态III、状态IVO呼吸和残余氧消耗进行高通量和高分辨率线粒体特异性测量。
Mitochondria are involved in generating more than 90 percent of cellular energy and are responsible for many cellular processes such as metabolism, cell signalling, apoptosis and ageing. Currently, there are a number of different experimental approaches employed to measure mitochondrial health and function. Here, we demonstrate a novel approach that quantifies substrate induced mitochondrial respiration from Drosophila. This protocol is optimized for mitochondria isolated from third instar larvae, and can also be used for mitochondria isolated from adult thoraces. This procedure outlines how to perform high throughput and high resolution mitochondria specific measurements for state II, state III, state IVO respiration and residual oxygen consumption.