Proteomics analysis of cytokine-induced dysfunction and death in insulin-producing INS-1E cells
Proteomics analysis of cytokine-induced dysfunction and death in insulin-producing INS-1E cells
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DOI：
10.1074/mcp.m700085-mcp200
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发表时间：
2007-12-01
影响因子：
7
通讯作者：
Mathieu, Chantal
中科院分区：
文献类型：
--
作者：
D'Hertog, Wannes;Overbergh, Lut;Mathieu, Chantal
胰岛浸润免疫细胞释放的细胞因子在 1 型糖尿病发病机制和胰岛移植后的 β 细胞功能障碍和细胞凋亡中发挥着至关重要的作用。 RNA研究揭示了在β细胞攻击过程中基因被激活或抑制的复杂途径。本研究的目的是使用二维 DIGE 分析体外暴露于炎症细胞因子的胰岛素产生 INS-1E 细胞中的蛋白质变化。在两个不同的 pH 范围内，我们观察到 2214 +/- 164 ( pH 4-7) 和 1641 +/- 73 ( pH 6-9) 个点。三个不同时间点（细胞因子暴露的 1、4 和 24 小时）的分析表明，主要变化仅在 24 小时后发生。此时，158 个蛋白质的表达发生改变（4.1%，n = 4，p
Cytokines released by islet-infiltrating immune cells play a crucial role in beta-cell dysfunction and apoptotic cell death in the pathogenesis of type 1 diabetes and after islet transplantation. RNA studies revealed complex pathways of genes being activated or suppressed during this beta-cell attack. The aim of the present study was to analyze protein changes in insulin-producing INS-1E cells exposed to inflammatory cytokines in vitro using two-dimensional DIGE. Within two different pH ranges we observed 2214 +/- 164 ( pH 4-7) and 1641 +/- 73 ( pH 6-9) spots. Analysis at three different time points ( 1, 4, and 24 h of cytokine exposure) revealed that the major changes were taking place only after 24 h. At this time point 158 proteins were altered in expression (4.1%, n = 4, p