Variation in Restriction Fragment Length Polymorphisms among Serial Isolates from Patients withTrichophyton rubrum Infection
Variation in Restriction Fragment Length Polymorphisms among Serial Isolates from Patients withTrichophyton rubrum Infection
复制标题
红色毛癣菌感染患者系列分离株限制性片段长度多态性的变异
DOI：
10.1128/jcm.39.9.3260-3266.2001
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发表时间：
2001
影响因子：
9.4
通讯作者：
R. Summerbell
中科院分区：
文献类型：
--
作者：
Aditya K. Gupta;Y. Kohli;R. Summerbell
红色毛癣菌和毛癣菌的分子分型研究从接受抗真菌治疗的患者和随访期间获得连续样品，进行来自患有脚趾甲真菌病的患者的须癣菌以确定真菌基因型是否随着时间的推移而变化。66株T. rubrum和11株T. 20例患者（16例T. rubrum，T.这些患者接受口服抗真菌治疗，并观察长达36个月。通过EcoRI消化的基因组DNA与从小亚基（18S）核糖体DNA和邻近的内部转录间隔区扩增的探针杂交来筛选这些菌株的遗传变异。对66株弓形虫进行了5种限制性片段长度多态性（RFLP）分析。红色。两个主要的RFLP类型，区分由一个带移，代表68%的样品。没有一个患者具有独特的基因型。在1年、2年或3年的时间内，经常从单个患者（同一指甲）中观察到一种以上的RFLP类型，即使在任何时候都没有出现治愈的病例中也是如此。从同一患者的不同指甲中提取的样本具有相同或不同的基因型。基因型变异不对应于任何可检测的表型变异。此外，未观察到给药疗效与观察到的基因型之间的相关性。而所研究的DNA区域在T. rubrum，T. mentagrophytes和T. tonsurans的种内RFLP变异，观察到的T。rubrum和T.须癣菌属菌株。虽然独立的多重感染和多个菌株的共存可以解释指甲中不同基因型的存在，但微进化事件，如快速亚株改组，如念珠菌属重复区域的研究中所见，也可能产生相同的结果。在未治愈患者的连续采样过程中回收多种菌株进一步表明分型系统无法区分复发或再感染、持续感染和新发感染。
ABSTRACT Molecular genotyping of strains of Trichophyton rubrumand T. mentagrophytes from patients with onychomycosis of the toes was performed to ascertain whether the fungal genotype changes over the course of time as sequential samples were obtained from patients receiving antifungal therapy and during follow-up. Sixty-six serial strains of T. rubrum and 11 strains of T. mentagrophytes were obtained from 20 patients (16 patients with T. rubrum, 4 with T. mentagrophytes) who were treated with oral antifungal therapy and observed over periods of up to 36 months. These strains were screened for genetic variation by hybridization of EcoRI-digested genomic DNAs with a probe amplified from the small-subunit (18S) ribosomal DNA and adjacent internal transcribed spacer regions. A total of five restriction fragment length polymorphism (RFLP) types were observed among 66 strains of T. rubrum. Two major RFLP types, differentiated by one band shift, represented 68% of the samples. None of the patients had a unique genotype. More than one RFLP type was often observed from a single patient (same nail) over a period of 1, 2, or 3 years, even in cases that did not appear cured at any time. Samples taken from different nails of the same patient had either the same or a different genotype. The genotypic variation did not correspond to any detectable phenotypic variation. Furthermore, no correlation was observed between the efficacy of the treatment administered and the genotype observed. While the DNA region studied distinguished among T. rubrum, T. mentagrophytes, and T. tonsurans, intraspecific RFLP variation was observed for T. rubrum andT. mentagrophytes strains. While independent multiple infection and coinhabitation of multiple strains may explain the presence of different genotypes in a nail, microevolutionary events such as rapid substrain shuffling, as seen in studies of repetitive regions in Candida species, may also produce the same result. The recovery of multiple strains during the course of sequential sampling of uncured patients further suggests that the typing system is not able to distinguish between relapse or reinfection, ongoing infection, and de novo infection.