Combination of bFGF, heparin and laminin induce the generation of dopaminergic neurons from rat neural stem cells both in vitro and in vivo
Combination of bFGF, heparin and laminin induce the generation of dopaminergic neurons from rat neural stem cells both in vitro and in vivo
复制标题
bFGF、肝素和层粘连蛋白的组合在体外和体内诱导大鼠神经干细胞产生多巴胺能神经元
DOI：
10.1016/j.jns.2007.01.076
复制
发表时间：
2007-04-15
影响因子：
4.4
通讯作者：
Wen, Tieqiao
中科院分区：
文献类型：
--
作者：
Yu, Yiqun;Gu, Shuting;Wen, Tieqiao
神经干细胞具有自我更新和多向分化潜能，可分化为神经元、星形胶质细胞和少突胶质细胞。由于这些特性，神经干细胞被认为是一个理想的候选人，临床目的，而细胞替代疗法治疗神经疾病的研究已被广泛研究，最近。在这篇文章中，我们展示了一种新的和有效的方法，诱导产生增殖的多巴胺能神经元从大鼠神经干细胞在碱性成纤维细胞生长因子，肝素和层粘连蛋白的存在下，在体外和体内。这些细胞在6-羟基多巴胺（6-OHDA）损伤的大鼠体内存活至少1个月。更重要的是，在移植后第30天观察到向封闭宿主组织的迁移。在这方面，我们预计这项技术可能会推进基于干细胞的治疗，以取代神经损伤或神经退行性疾病中丢失的神经元。(c)2007 Elsevier B. V.保留所有权利。
Neural stem cells (NSCs) are self-renewing and pluripotent, which can differentiate into neurons, astrocytes and oligodendrocytes. Due to these properties, NSCs are supposed to be an ideal candidate to clinical purpose while research on cell replacement therapy to treat neural diseases has been widely investigated recently. In this article, we demonstrated a new and efficient method to induce the generation of proliferative dopaminergic neurons from rat NSCs in the presence of bFGF, heparin and laminin both in vitro and in vivo. These cells were testified to survive in the grafted 6-Hydroxy-Dopamine (6-OHDA) lesioned rat for at least 1 month. More importantly, migration to close host tissue was observed on day 30 post-transplantation. In this regard, we anticipated that this technology may advance stem cell-based therapy to replace lost neurons in neural injury or neurodegenerative disorders. (c) 2007 Elsevier B.V. All rights reserved.