INCREASES IN P11 AND ANNEXIN II PROTEINS CORRELATE WITH DIFFERENTIATION IN THE PC12 PHEOCHROMOCYTOMA
INCREASES IN P11 AND ANNEXIN II PROTEINS CORRELATE WITH DIFFERENTIATION IN THE PC12 PHEOCHROMOCYTOMA
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DOI：
10.1016/0006-291x(91)90666-u
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发表时间：
1991-06-28
影响因子：
3.1
通讯作者：
HUGHES, JP
中科院分区：
文献类型：
--
作者：
FOX, MT;PRENTICE, DA;HUGHES, JP
在PC 12大鼠肾上腺嗜铬细胞瘤细胞中，用免疫印迹分析法检测神经生长因子、星形孢菌素和表皮生长因子对p11和膜联蛋白II的调节作用。已知神经生长因子可诱导PC 12细胞中的神经突生长，刺激p11增加5倍，并且较高水平的p11是暴露于神经生长因子长达10天的PC 12细胞的特征。神经生长因子诱导膜联蛋白II更大幅度增加（13.6倍）。Staurosporine是一种蛋白激酶抑制剂，在高浓度下诱导神经突形成，在增加细胞内p11和膜联蛋白II水平方面与神经生长因子一样有效。表皮生长因子的效果不如神经生长因子和星形孢菌素，仅使p11增加2倍，膜联蛋白II增加3倍。表皮生长因子在增加细胞内p11和膜联蛋白II水平的无效性与表皮生长因子不刺激PC 12细胞中神经突生长的事实一致。这里提出的证据表明，p11和/或膜联蛋白II可能在PC 12细胞分化中发挥作用。
Regulation of p11 and annexin II by nerve growth factor, staurosporine, and epidermal growth factor was examined in PC12 rat adrenal pheochromocytoma cells using immunoblot analysis. Nerve growth factor, which is known to induce neurite outgrowth in PC12 cells, stimulated a five-fold increase in p11 and the higher levels of p11 were characteristic of PC12 cells exposed to nerve growth factor for up to ten days. Nerve growth factor induced an even greater increase (13.6-fold) in annexin II. Staurosporine, a protein kinase inhibitor that at high concentrations induces neurite formation, was as effective as nerve growth factor in increasing the intracellular levels of p11 and annexin II. Epidermal growth factor was less effective than nerve growth factor and staurosporine, producing only a two-fold increase in p11 and a three-fold increase in annexin II. The ineffectiveness of epidermal growth factor in increasing intracellular levels of p11 and annexin II is consistent with the fact that epidermal growth factor does not stimulate neurite outgrowth in PC12 cells. Evidence presented here suggests that p11 and/or annexin II may play a role in PC12 cell differentiation.