A novel methicillin-resistance cassette in community- acquired Methicillin-resistant Staphylococcus aureus isolates of diverse genetic backgrounds
A novel methicillin-resistance cassette in community- acquired Methicillin-resistant Staphylococcus aureus isolates of diverse genetic backgrounds
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DOI：
10.1086/344326
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发表时间：
2002-11-01
影响因子：
6.4
通讯作者：
Boyle-Wang, S
中科院分区：
文献类型：
--
作者：
Daum, RS;Ito, T;Boyle-Wang, S
直到最近，还不清楚社区获得性（CA）耐甲氧西林金黄色葡萄球菌（MRSA）分离株是否代表医院MRSA分离株在社区中的传播。在2个CA-MRSA分离株中，发现了一种新的遗传元件，命名为葡萄球菌盒式染色体mec（SCCmec）IV型;它与SCCmec I-III型的不同之处在于其尺寸小且不存在非β-内酰胺遗传耐药决定簇。为了研究IV型SCCmec的患病率，对12株CA-MRSA分离株的DNA进行了SCCmec的聚合酶链反应表征。这12株CA-MRSA分离株来自不同的遗传背景，其分层分为5个脉冲场凝胶电泳型，4个凝固酶型和2个核糖体型。12个分离株中有11个含有新的SCCmec IV型元件。10例仅对β-内酰胺类抗生素耐药。SCCmec IV型存在于CA-MRSA分离株的基因组上。它相对较小的大小和存在于不同遗传背景的分离株中表明它可能在S.金黄色葡萄球菌分离株。
Until recently, it has been unclear whether community-acquired (CA) methicillin-resistant Staphylococcus aureus (MRSA) isolates represent the spread of hospital MRSA isolates into the community. In 2 CA-MRSA isolates, a novel genetic element, designated staphylococcal cassette chromosome mec (SCCmec) type IV, was found; it differs from SCCmec types I-III in its small size and absence of non-beta-lactam genetic-resistance determinants. To study the prevalence of type IV SCCmec, polymerase chain reaction characterization of SCCmec was performed on DNA from 12 CA-MRSA isolates. The 12 CA-MRSA isolates were from diverse genetic backgrounds, as evidenced by their stratification into 5 pulsed-field gel electrophoresis types, 4 coagulase types, and 2 ribotypes. Eleven of the 12 isolates contained the novel SCCmec type IV element. Ten were resistant only to beta-lactam antibiotics. SCCmec type IV is present on the genome of CA-MRSA isolates. Its relatively small size and presence in isolates of diverse genetic backgrounds suggest that it may spread among S. aureus isolates.