HpSS: A new silver staining method for Helicobacter pylori
HpSS: A new silver staining method for Helicobacter pylori
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DOI：
10.1136/jcp.50.6.461
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发表时间：
1997-06-01
影响因子：
3.4
通讯作者：
Barbareschi, M
中科院分区：
文献类型：
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作者：
Doglioni, C;Turrin, M;Barbareschi, M
目的 - 验证所提出的新银染方法在检测胃活检中幽门螺杆菌方面是否优于其他成熟的方法。方法 - 来自 70 名连续未选择的患者的 140 对胃窦和胃底活检，常规福尔马林固定和石蜡包埋，用苏木精和伊红、改良吉姆萨和拟议的幽门螺杆菌银染色 (HpSS) 进行染色。用抗H pylori多克隆抗血清在同一材料中进行H pylori免疫检测。结果-苏木精和伊红检测48例患者的89个活检组织中检测到H pylori；用吉姆萨染色进一步进行五次活检（一次胃窦和四次胃底），从而识别出另一名幽门螺杆菌感染患者。新的银染方法在这两种方法检测到的所有病例中均呈阳性，并额外检测到三名感染患者（另外五名活检呈阳性）。免疫组织化学又检测到一例其他任何方法都未发现的阳性病例（两个阳性活检）。结论-提出的 HpSS 方法在检测幽门螺杆菌方面高度敏感；它很简单，并且与常规用于评估胃活检中幽门螺杆菌的其他方法相比效果很好。
Aims-To verify whether the proposed new silver staining method compares favourably with other well established methods in the detection of Helicobacter pylori in gastric biopsies.Methods-One hundred and forty pairs of antral and fundic biopsies, routinely formalin fixed and paraffin wax embedded, from 70 consecutive unselected patients were stained with haematoxylin and eosin, modified Giemsa, and the proposed H pylori silver stain (HpSS). H pylori immunodetection was performed in the same material with a polyclonal antiserum against H pylori.Results-H pylori was detected in 89 biopsies from 48 patients with haematoxylin and eosin; in a further five biopsies (one antral and four fundic) with Giemsa stain, thereby identifying one more H pylori infected patient. The new silver staining method was positive in all the cases detected by these two methods and detected three extra infected patients (five more positive biopsies). Immunohistochemistry detected one more positive case (two positive biopsies) not identified by any of the other methods.Conclusions-The HpSS method proposed is highly sensitive in detecting H pylori; it is simple and it compares well with other methods used routinely for evaluating gastric biopsies for H pylori.