Regulation of area identity in the mammalian neocortex by Emx2 and Pax6
Regulation of area identity in the mammalian neocortex by Emx2 and Pax6
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DOI：
10.1126/science.288.5464.344
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发表时间：
2000-04-14
期刊：
影响因子：
56.9
通讯作者：
O'Leary, DDM
中科院分区：
文献类型：
--
作者：
Bishop, KM;Goudreau, G;O'Leary, DDM
外在和遗传机制在确定哺乳动物新皮质区域中的贡献一直是一个有争议的问题。本研究分析了调节基因 Emx2 和 Pax6 的作用，它们在新皮质心室区的特定区域中以相反的梯度表达。分子标记模式的变化以及犁沟和丘脑之间的区域特异性连接表明，Emx2 和 Pax6 突变小鼠中新皮质的区域化以相反的方式发生了不成比例的改变，这与它们的反梯度表达预测的相反：在 Emx2 突变体中，嘴部区域扩大，尾部区域收缩，而在 Pax6 突变体中看到相反的效果。这些发现表明 Emx2 和 Pax6 合作调节新皮质的区域化并赋予皮质细胞区域身份。
The contribution of extrinsic and genetic mechanisms in determining areas of the mammalian neocortex has been a contested issue. This study analyzes the roles of the regulatory genes Emx2 and Pax6, which are expressed in opposing gradients in the neocortical ventricular zone, in specifying areas. Changes in the patterning of molecular markers and area-specific connections between the colter and thalamus suggest that arealization of the neocortex is disproportionately altered in Emx2 and Pax6 mutant mice in opposing manners predicted from their countergradients of expression: rostral areas expand and caudal areas contract in Emx2 mutants, whereas the opposite effect is seen in Pax6 mutants. These findings suggest that Emx2 and Pax6 cooperate to regulate arealization of the neocortex and to confer area identity to cortical cells.