Production of core and virus-like particles with baculovirus infected insect cells.
Production of core and virus-like particles with baculovirus infected insect cells.
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DOI：
10.1007/3-540-45736-4_9
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
L. Maranga;P. Cruz;J. Auniņš;M. Carrondo
中科院分区：
文献类型：
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作者：
L. Maranga;P. Cruz;J. Auniņš;M. Carrondo
本文综述了利用杆状病毒感染昆虫细胞生产核心颗粒和病毒样颗粒的工艺开发的基本方面。所涉及的问题包括：颗粒形成和单体组成、用于最佳细胞生长的化学和物理条件、杆状病毒复制和产物表达、感染策略的多样性和方法的放大。研究感染和未感染细胞代谢需求的差异对于高细胞密度过程是必要的。在生物反应器中，比氧摄取速率（OURsp）在工艺放大中起着核心作用，导致生物反应器操作参数的规格。剪切应力也可以是生物反应器操作的重要变量，因为其影响细胞生长和产物表达。
In this paper the fundamental aspects of process development for the production of core and virus-like particles with baculovirus infected insect cells are reviewed. The issues addressed include: particle formation and monomer composition, chemical and physical conditions for optimal cell growth, baculovirus replication and product expression, multiplicity of infection strategy, and scale-up of the process. Study of the differences in the metabolic requirements of infected and non-infected cells is necessary for high cell density processes. In the bioreactor, the specific oxygen uptake rate (OURsp) plays a central role in process scale-up, leading to the specification of the bioreactor operational parameters. Shear stress can also be an important variable for bioreactor operation due to its influence on cell growth and product expression.