Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera.
Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera.
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DOI：
10.1016/j.ab.2009.09.041
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发表时间：
2010-02-01
影响因子：
2.9
通讯作者：
Aswad DW
中科院分区：
文献类型：
--
作者：
Khoury MK;Parker I;Aswad DW
我们发现，使用全画幅CMOS传感器的某些中档消费级数码单反相机在从使用增强化学发光检测的免疫印迹中获取信号方面优于X射线胶片。这些照相机的灵敏度可与X射线胶片相媲美，但线性动态范围增加了3倍，随着时间的推移节省了大量成本，使用更方便，并消除了与处理胶片相关的化学废物。
We found that certain mid-range consumer level digital SLR cameras using full-frame CMOS sensors outperform x-ray film in acquiring signals from immunoblots that use enhanced chemiluminescence for detection. These cameras exhibit a sensitivity comparable to x-ray film, yet provide a 3-fold increase in linear dynamic range and substantial cost saving over time, are more convenient to use, and eliminate the chemical waste associated with processing film.