Phosphorylation of branched-chain 2-oxo acid dehydrogenase complex in isolated hepatocytes.

Phosphorylation of branched-chain 2-oxo acid dehydrogenase complex in isolated hepatocytes.
复制标题

分离肝细胞中支链 2-含氧酸脱氢酶复合物的磷酸化。

DOI:
10.1016/s0006-291x(87)80012-8
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发表时间:
1987
影响因子:
3.1
通讯作者:
S. Yeaman
S. Yeaman
中科院分区:
生物学4区
文献类型:
--
作者:
S. Jones;A. Sim;D. Hardie;S. Yeaman

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从喂食低蛋白食物的大鼠中分离肝细胞,用[32P] pip孵育并分析磷酸化蛋白。支链2-氧酸脱氢酶复合物El抗体免疫沉淀显示El α-亚基磷酸化。α-亚基的胰蛋白酶肽分析表明,两个位点被磷酸化。4- 2-氧戊酸甲酯和dl -2-氯4-甲基戊酸甲酯减少了这两个位点的标记。几种激素对支链2-氧酸脱氢酶的磷酸化没有明显的直接影响。
Hepatocytes, isolated from rats fed a low-protein diet, were incubated with [32P]Piand the phosphoproteins analysed. Immunoprecipitation using antibody against El of branched-chain 2-oxo acid dehydrogenase complex demonstrated phosphorylation of the α-subunit of El. Analysis of the tryptic phosphopeptides from the α-subunit indicated that two sites were phosphorylated. 4-methyl 2-oxopentanoate and DL-2-chloro 4-methylpentanoate decreased labelling of both sites. No major direct effects of several hormones on phosphorylation of branched-chain 2-oxo acid dehydrogenase was observed.
生长激素对离体大鼠肝细胞蛋白质磷酸化的影响。
DOI: 10.1021/bi00377a009
发表时间: 1987
期刊: Biochemistry
影响因子: 2.9
作者:
Yamada,K;Lipson,KE;Marino,MW;Donner,DB
通讯作者: Donner,DB
DOI: 10.1016/0003-9861(84)90361-8
发表时间: 1984-01-01
影响因子: 3.9
作者:
PAXTON, R;HARRIS, RA
通讯作者: HARRIS, RA
从低蛋白饮食喂养的大鼠中分离出的肝细胞中支链 2-含氧酸脱氢酶复合物的调节。
DOI: 10.1042/bj2340285
发表时间: 1986
期刊: The Biochemical journal
影响因子: --
作者:
Harris,RA;Paxton,R;Goodwin,GW;Powell,SM
通讯作者: Powell,SM
DOI: 10.1016/s0006-291x(82)80168-x
发表时间: 1982
影响因子: 3.1
作者:
Harris,RA;Paxton,R;Parker,RA
通讯作者: Parker,RA