Gelatin-based photonic hydrogels for visual detection of pathogenic Pseudomonas aeruginosa
Gelatin-based photonic hydrogels for visual detection of pathogenic Pseudomonas aeruginosa
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DOI：
10.1016/j.snb.2020.129137
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发表时间：
2021-01-17
影响因子：
8.4
通讯作者：
Zhu, Jintao
中科院分区：
文献类型：
--
作者：
Gao, Yujie;Chen, Yu;Zhu, Jintao
病原微生物的鉴定或检测是预防和/或治疗细菌感染的最重要方面之一。在这项研究中，我们报告了一种简单而通用的策略，用于制备基于明胶的光子水凝胶传感器，用于铜绿假单胞菌（P. aeruginosa）的视觉检测。通过丙烯酰胺（AM）、N，N '-亚甲基双-（丙烯酰胺）（BIS）和明胶甲基丙烯酸酯（GelMA）的原位光聚合，制备了具有自组装一维链状结构的碳包覆Fe 3 O 4纳米粒子（Fe3O4@C NPs）。在铜绿假单胞菌存在下，明胶酶引发的明胶水解降低了光子水凝胶的交联密度，使水凝胶体积膨胀，增加了胶体颗粒的晶格间距，并使光子水凝胶的反射光谱红移。由于反射峰的移动，可以用肉眼观察到光子水凝胶的光学外观变化。此外，我们发现光子水凝胶在大肠杆菌（E。大肠杆菌），表明它们通过简单的结构颜色变化来区分各种细菌的潜力。这一发现为用于铜绿假单胞菌视觉检测的比色传感器提供了概念验证，并证明了光子水凝胶在病原体视觉检测中的巨大潜力。
Identification or detection of pathogenic microbes is one of the most important aspects to prevent and/or treat bacterial infection. In this study, we report a facile yet versatile strategy for preparation of gelatin-based photonic hydrogel sensors for visual detection of Pseudomonas aeruginosa (P. aeruginosa). The photonic hydrogels are generated by in-situ photopolymerization of acrylamide (AM), N, N'-methylenebis-(acrylamide) (BIS), and gelatin methacrylate (GelMA) with the self-assembled one-dimensional (1D) chain-like structures of carbon-encapsulated Fe3O4 nanoparticles (Fe3O4@C NPs). In the presence of P. aeruginosa, the hydrolysis of gelatin triggered by gelatinase reduces the crosslinking density of photonic hydrogel, expands the hydrogel in volume, increases the lattice spacing of the colloidal particles, and red-shifts the reflection spectra of the photonic hydrogel. The optical appearance variation of the photonic hydrogel, resulting from the reflection peak shift, can be observed by naked eyes. Besides, we show that the photonic hydrogels exhibit negligible appearance changes with the presence of Escherichia coli (E. coli), indicating their potential for distinguishing various bacteria by a simple structural color variation. This finding provides a proof-of-concept for a colorimetric sensor for visual detection of P. aeruginosa and demonstrates the great potential of the photonic hydrogels in visual detection of pathogens.