Endonuclease cleavage of messenger RNA in Bacillus subtilis
Endonuclease cleavage of messenger RNA in Bacillus subtilis
复制标题
DOI：
10.1046/j.1365-2958.2002.02830.x
复制
发表时间：
2002-03-01
影响因子：
3.6
通讯作者：
Bechhofer, DH
中科院分区：
文献类型：
--
作者：
Drider, D;DiChiara, JM;Bechhofer, DH
构建ermC基因的缺失衍生物，其表达254个核苷酸的mRNA。这种mRNA的小尺寸便于检测在尺寸上与全长转录物没有很大差异的加工产物。在红霉素的存在下，其诱导ermC mRNA的5 '端附近的核糖体停滞，254个核苷酸的mRNA在核糖体停滞的位点被内切核酸裂解。只有下游产品的这种切割是可检测的;上游产品显然是太不稳定而无法检测。在加入利福平后，下游切割产物有时积累，表明5'端的停滞核糖体赋予该RNA片段稳定性。Bs-RNase III和RNase M5（枯草杆菌的两种已知窄特异性内切核糖核酸酶）都不负责这种切割。这些结果表明存在于B中。枯草芽孢杆菌的另一种特异性核糖核酸内切酶，其可以是核糖体相关的。
A deletion derivative of the ermC gene was constructed that expresses a 254-nucleotide mRNA. The small size of this mRNA facilitated the detection of processing products that did not differ greatly in size from the full-length transcript. In the presence of erythromycin, which induces ribosome stalling near the 5'end of ermC mRNA, the 254-nucleotide mRNA was cleaved endonucleolytically at the site of ribosome stalling. Only the downstream product of this cleavage was detectable; the upstream product was apparently too unstable to be detected. The downstream cleavage product accumulated at times after rifampicin addition, suggesting that the stalled ribosome at the 5' end conferred stability to this RNA fragment. Neither Bs-RNase III nor RNase M5, the two known narrow-specificity endoribonucleases of Bacillus subtilis, was responsible for this cleavage. These results indicate the presence in B. subtilis of another specific endoribonuclease, which may be ribosome associated.