Role of liver fatty acid binding protein in hepatocellular injury: Effect of CrPic treatment
Role of liver fatty acid binding protein in hepatocellular injury: Effect of CrPic treatment
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DOI：
10.1016/j.jinorgbio.2013.03.015
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发表时间：
2013-07-01
影响因子：
3.9
通讯作者：
Yan，Peng
中科院分区：
文献类型：
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作者：
Fan，Weijiang;Chen，Kun;Yan，Peng
本研究旨在研究吡啶甲酸铬（CrPic，图1）对四氧嘧啶诱导的肝损伤的肝保护活性的分子机制。四氧嘧啶诱发小鼠糖尿病并伴有肝损伤。在这项研究中，我们研究了CrPic治疗对肝损伤的保护作用以及肝脏脂肪酸结合蛋白在早期肝细胞损伤诊断中的信号作用。在本研究中，四氧嘧啶组的丙氨酸氨基转移酶（ALT; EC 2.6.1.2）和天冬氨酸氨基转移酶（AST; EC 2.6.1.1）水平分别比对照组高71%和50%（ALT：14.51±0.74; AST：22.60±0.69）。CrPic组的AST和ALT水平与对照组相比差异极小。在此，CrPic表现出改善四氧嘧啶诱导的小鼠肝脏氧化应激。观察到肝脏脂肪酸结合蛋白（L-FABP）显著增加，这表明肝脏组织中脂肪酸利用增加[1]。在这项研究中，与四氧嘧啶组相比，对照组（1.1倍）和CrPic组（0.78倍）中L-FABP的mRNA水平均增加。这些结果表明，肝损伤可能是预防的CrPic，是一个潜在的目标，用于治疗早期肝损伤。
This study was designed to investigate the molecular mechanisms of chromium picolinate (CrPic, Fig. 1) hepatoprotective activity from alloxan-induced hepatic injury. Diabetes is induced by alloxan-treatment concurrently with the hepatic injury in mice. In this study, we investigate the protective effect of CrPic treatment in hepatic injury and the signal role of liver fatty acid binding protein in early hepatocellular injury diagnostics. In this study, alanine aminotransferase (ALT; EC 2.6.1.2) and aspartate aminotransferase (AST; EC 2.6.1.1) levels in the alloxan group were higher 71% and 50%, respectively, than those of the control group (ALT: 14.51±0.74; AST: 22.60±0.69). The AST and ALT levels in CrPic group were of minimal difference compared to the control groups. Here, CrPic exhibited amelioration alloxan induced oxidative stress in mouse livers. A significant increase in liver fatty acid-binding protein (L-FABP) was observed, which indicates increased fatty acid utilization in liver tissue [1]. In this study, the mRNA levels of L-FABP increased in both the control (1.1 fold) and CrPic (0.78 fold) groups compared the alloxan group. These findings suggest that hepatic injury may be prevented by CrPic, and is a potential target for use in the treatment of early hepatic injury.