FC-EPSILON-RI-MEDIATED TYROSINE PHOSPHORYLATION AND ACTIVATION OF THE 72-KDA PROTEIN-TYROSINE KINASE, PTK72, IN RBL-2H3 RAT-TUMOR MAST-CELLS
FC-EPSILON-RI-MEDIATED TYROSINE PHOSPHORYLATION AND ACTIVATION OF THE 72-KDA PROTEIN-TYROSINE KINASE, PTK72, IN RBL-2H3 RAT-TUMOR MAST-CELLS
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DOI：
10.1073/pnas.89.19.9107
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发表时间：
1992-10-01
影响因子：
11.1
通讯作者：
OLIVER, JM
中科院分区：
文献类型：
--
作者：
HUTCHCROFT, JE;GEAHLEN, RL;OLIVER, JM
在RBL-2H3大鼠肿瘤肥大细胞中，高亲和力IgE受体FcepsilonRI交联可导致多种蛋白的酪氨酸磷酸化。这些磷酸化蛋白包括磷脂酶C(γ)1、FcepsilonRI的β和γ亚基、Src家族蛋白酪氨酸激酶Lyn和一种72 kda的蛋白，该蛋白从抗原刺激细胞的裂解物中与受体β亚基抗体共免疫沉淀。我们现在提出的证据表明，72-kDa fcepsilonri相关蛋白是蛋白酪氨酸激酶PTK72，它在B淋巴细胞中形成抗原受体复合物的一部分。该鉴定是基于抗ptk72抗血清的免疫反应性、亲和树脂肝素/琼脂糖的色谱图谱和一维磷酸肽定位研究。从抗原活化的RBL-2H3细胞裂解物制备的抗ptk72免疫复合物中，该激酶的酶活性增加。72kda蛋白酪氨酸激酶是RBL-2H3细胞抗酪氨酸免疫沉淀物中体外酪氨酸磷酸化的主要底物。RBL-2H3肥大细胞与B淋巴细胞共享一个受体激活的蛋白酪氨酸激酶PTK72，这一发现为多链免疫识别受体启动的共同信号通路的存在提供了额外的支持。
In RBL-2H3 rat tumor mast cells, crosslinking the high-affinity IgE receptor FcepsilonRI causes tyrosine phosphorylation of multiple proteins. These phosphoproteins include phospholipase C(gamma)1, the beta and gamma subunits of the FcepsilonRI, the Src family protein-tyrosine kinase Lyn, and a 72-kDa protein that coimmunoprecipitates from lysates of antigen-stimulated cells with antibody to the receptor beta subunit. We now present evidence that the 72-kDa FcepsilonRI-associated protein is the protein-tyrosine kinase PTK72 that forms part of the antigen receptor complex in B lymphocytes. The identification is based on immunoreactivity with anti-PTK72 antiserum, chromatographic profiles on the affinity resin heparin/agarose, and one-dimensional phosphopeptide mapping studies. Enzymatic activity of the kinase is increased in anti-PTK72 immune complexes prepared from lysates of antigen-activated RBL-2H3 cells. The 72-kDa protein-tyrosine kinase is the principal substrate for in vitro tyrosine phosphorylation in anti-phosphotyrosine immunoprecipitates of RBL-2H3 cells. The discovery that RBL-2H3 mast cells share a receptor-activated protein-tyrosine kinase, PTK72, with B lymphocytes provides additional support for the existence of common signaling pathways initiated by multichain immune recognition receptors.