The sea urchin (Strongylocentrotus purpuratus) test and spine proteomes
The sea urchin (Strongylocentrotus purpuratus) test and spine proteomes
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DOI：
10.1186/1477-5956-6-22
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发表时间：
2008-08-11
期刊：
影响因子：
2
通讯作者：
Mann, Matthias
中科院分区：
文献类型：
--
作者：
Mann, Karlheinz;Poustka, Albert J.;Mann, Matthias
背景：生物矿物的有机基质在生物矿物的形成过程中起着重要的作用，并决定着生物矿物的性质。然而，目前大多数生物矿物基质的成分仍然未知。海胆是发育生物学和生物矿化的重要模式生物，在蛋白质水平上，只有少数基质成分被鉴定和表征。最近出版的球海胆的基因组序列呈现可能不仅在基因水平上的可能的基质蛋白的识别，但也直接识别的蛋白质中包含的基质的骨骼elements.Results的蛋白质组学分析的深入，高精度，我们确定了110蛋白质的海胆测试和脊柱有机matrix的组件。这些蛋白质中有四十种发生在两个隔室中，而其他蛋白质则是其各自隔室所特有的。首次在海胆骨骼基质中检测到超过95%的蛋白质。最丰富的蛋白质在这两个矩阵是以前的特征针状基质蛋白SM 50，但至少有八个其他成员，这组，其中许多人只知道作为概念的翻译产物以前，通过质谱序列分析的肽来源于体外基质降解。基质还含有与生物矿化过程有关的蛋白质，这些蛋白质先前通过使用抗体或特异性酶抑制剂（例如基质金属蛋白酶和间充质特异性MSP 130家族的成员）的抑制研究而被发现。其他成分是碳酸酐酶，胶原蛋白，棘连蛋白，α 2-巨球蛋白样蛋白和几种含有清道夫受体富含半胱氨酸结构域的蛋白质。几个可能的信号转导途径的组成部分，如GTP结合蛋白，一个semaphorin和一个可能的酪氨酸kinase也identified.Conclusion：本报告提出了目前最全面的名单海胆骨骼基质蛋白。在海胆骨架基质中发现的复杂蛋白质混合物可能反映了矿化过程的许多不同方面。由于基于LC-MS/MS的方法直接测量肽，因此我们的结果验证了许多预测的基因并证实了相应蛋白质的存在。考虑到许多新鉴定的基质蛋白，蛋白质组学研究可能作为一个重要的模式生物的生物矿化过程的进一步探索的路线图。
Background: The organic matrix of biominerals plays an important role in biomineral formation and in determining biomineral properties. However, most components of biomineral matrices remain unknown at present. In sea urchin, which is an important model organism for developmental biology and biomineralization, only few matrix components have been identified and characterized at the protein level. The recent publication of the Strongylocentrotus purpuratus genome sequence rendered possible not only the identification of possible matrix proteins at the gene level, but also the direct identification of proteins contained in matrices of skeletal elements by in-depth, high-accuracy, proteomic analysis.Results: We identified 110 proteins as components of sea urchin test and spine organic matrix. Fourty of these proteins occurred in both compartments while others were unique to their respective compartment. More than 95% of the proteins were detected in sea urchin skeletal matrices for the first time. The most abundant protein in both matrices was the previously characterized spicule matrix protein SM50, but at least eight other members of this group, many of them only known as conceptual translation products previously, were identified by mass spectrometric sequence analysis of peptides derived from in vitro matrix degradation. The matrices also contained proteins implicated in biomineralization processes previously by inhibition studies using antibodies or specific enzyme inhibitors, such as matrix metalloproteases and members of the mesenchyme-specific MSP130 family. Other components were carbonic anhydrase, collagens, echinonectin, a alpha 2-macroglobulin-like protein and several proteins containing scavenger receptor cysteine-rich domains. A few possible signal transduction pathway components, such as GTP-binding proteins, a semaphorin and a possible tyrosine kinase were also identified.Conclusion: This report presents the most comprehensive list of sea urchin skeletal matrix proteins available at present. The complex mixture of proteins identified in matrices of the sea urchin skeleton may reflect many different aspects of the mineralization process. Because LC-MS/MS-based methods directly measures peptides our results validate many predicted genes and confirm the existence of the corresponding proteins. Considering the many newly identified matrix proteins, this proteomic study may serve as a road map for the further exploration of biomineralization processes in an important model organism.