A chromosome conformation capture ordered sequence of the barley genome
A chromosome conformation capture ordered sequence of the barley genome
复制标题
染色体构象捕获大麦基因组的有序序列
DOI：
10.1038/nature22043
复制
发表时间：
2017-04-27
期刊：
影响因子：
64.8
通讯作者：
Stein, Nils
中科院分区：
文献类型：
--
作者：
Mascher, Martin;Gundlach, Heidrun;Stein, Nils
自农业出现以来，小麦族的禾本科牧草一直是温带地区的主要食物来源。它们的大基因组的特点是高含量的重复元素和大的着丝粒周围区域，几乎没有减数分裂重组。在这里，我们提出了一个高质量的大麦(Hordeum uggarL.)参考基因组组装。我们使用染色体构象捕捉图来推导整个着丝粒周围空间的序列的线性顺序，并研究在兆基分辨率下染色质在细胞核中的空间组织。基因和重复元件的组成在远端和近端是不同的。基因家族分析揭示了与营养物质向发育中的种子的运输和谷物中碳水化合物的动员有关的基因的谱系特异性复制。我们通过检查现代优良种质中序列变异的基因组划分，突出易受遗传侵蚀的区域，论证了大麦参考序列在育种中的重要性。
Cereal grasses of the Triticeae tribe have been the major food source in temperate regions since the dawn of agriculture. Their large genomes are characterized by a high content of repetitive elements and large pericentromeric regions that are virtually devoid of meiotic recombination. Here we present a high-quality reference genome assembly for barley (Hordeum vulgareL.). We use chromosome conformation capture mapping to derive the linear order of sequences across the pericentromeric space and to investigate the spatial organization of chromatin in the nucleus at megabase resolution. The composition of genes and repetitive elements differs between distal and proximal regions. Gene family analyses reveal lineage-specific duplications of genes involved in the transport of nutrients to developing seeds and the mobilization of carbohydrates in grains. We demonstrate the importance of the barley reference sequence for breeding by inspecting the genomic partitioning of sequence variation in modern elite germplasm, highlighting regions vulnerable to genetic erosion.