SCHENGEN receptor module drives localized ROS production and lignification in plant roots
SCHENGEN receptor module drives localized ROS production and lignification in plant roots
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DOI：
10.15252/embj.2019103894
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发表时间：
2020-03-18
期刊：
影响因子：
11.4
通讯作者：
Geldner, Niko
中科院分区：
文献类型：
--
作者：
Fujita, Satoshi;De Bellis, Damien;Geldner, Niko
NADPH氧化酶（NOXs）产生的活性氧（ROS）影响动物和植物中的许多过程，许多植物受体途径涉及ROS的快速、NOX依赖性增加。然而，它们的一般反应性使得确定ROS的精确作用和直接分子作用具有挑战性。在植物中，一个众所周知的ROS作用是为细胞壁中的木质素过氧化物酶提供共底物。木质素可以通过精确的空间控制沉积，但其潜在的机制仍然是难以捉摸的。在这里，我们建立了一个激酶信号传递，发挥直接的，空间控制ROS的生产和细胞壁内的木质化。我们发现，极性定位的一个单一的激酶组件是至关重要的通路功能。我们的数据表明，更广泛的本地化组件的交叉允许微米级精度的木质化，该系统是通过启动ROS生产作为一个关键的过氧化物酶共基板触发。
Production of reactive oxygen species (ROS) by NADPH oxidases (NOXs) impacts many processes in animals and plants, and many plant receptor pathways involve rapid, NOX-dependent increases of ROS. Yet, their general reactivity has made it challenging to pinpoint the precise role and immediate molecular action of ROS. A well-understood ROS action in plants is to provide the co-substrate for lignin peroxidases in the cell wall. Lignin can be deposited with exquisite spatial control, but the underlying mechanisms have remained elusive. Here, we establish a kinase signaling relay that exerts direct, spatial control over ROS production and lignification within the cell wall. We show that polar localization of a single kinase component is crucial for pathway function. Our data indicate that an intersection of more broadly localized components allows for micrometer-scale precision of lignification and that this system is triggered through initiation of ROS production as a critical peroxidase co-substrate.