Reconstitution of EPA and DHA biosynthesis in arabidopsis: iterative metabolic engineering for the synthesis of n-3 LC-PUFAs in transgenic plants.
Reconstitution of EPA and DHA biosynthesis in arabidopsis: iterative metabolic engineering for the synthesis of n-3 LC-PUFAs in transgenic plants.
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DOI：
10.1016/j.ymben.2013.03.001
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发表时间：
2013-05
影响因子：
8.4
通讯作者：
Sayanova, Olga
中科院分区：
文献类型：
--
作者：
Ruiz-Lopez, Noemi;Haslam, Richard P.;Usher, Sarah L.;Napier, Johnathan A.;Sayanova, Olga
关键词：
在拟南芥中进行了优化转基因植物中非天然长链多不饱和脂肪酸积累的迭代方法。系统地确定了许多不同转基因酶活性的贡献，以及内源性脂肪酸代谢的贡献。通过中性、极性和酰基-CoA池的脂质组学分析告知连续迭代。这种方法允许在先前报道的拟南芥种子中二十碳五烯酸积累水平上提高四倍，并且还促进了高价值多不饱和脂肪酸二十二碳六烯酸的成功工程化至10倍更高的水平。我们的研究确定了指导转基因籽油中这些脂肪酸有效合成所需的最小基因集。在转基因植物中成功重复EPA和DHA积累。系统鉴定DHA积累的最佳基因。脂质组学驱动的植物代谢工程的实用性的证明。
An iterative approach to optimising the accumulation of non-native long chain polyunsaturated fatty acids in transgenic plants was undertaken in Arabidopsis thaliana. The contribution of a number of different transgene enzyme activities was systematically determined, as was the contribution of endogenous fatty acid metabolism. Successive iterations were informed by lipidomic analysis of neutral, polar and acyl-CoA pools. This approach allowed for a four-fold improvement on levels previously reported for the accumulation of eicosapentaenoic acid in Arabidopsis seeds and also facilitated the successful engineering of the high value polyunsaturated fatty acid docosahexaenoic acid to 10-fold higher levels. Our studies identify the minimal gene set required to direct the efficient synthesis of these fatty acids in transgenic seed oil. ► Successful iteration of EPA and DHA accumulation in transgenic plants. ► Systematic identification of optimal genes for accumulation of DHA. ► Demonstration of the utility of lipidomic-driven plant metabolic engineering.
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影响因子：
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