The large subunit of the mammalian mitochondrial ribosome - Analysis of the complement of ribosomal proteins present
The large subunit of the mammalian mitochondrial ribosome - Analysis of the complement of ribosomal proteins present
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DOI：
10.1074/jbc.m106510200
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发表时间：
2001-11-23
影响因子：
4.8
通讯作者：
Spremulli, LL
中科院分区：
文献类型：
--
作者：
Koc, EC;Burkhart, W;Spremulli, LL
哺乳动物线粒体核糖体大亚基（39 S）的所有蛋白质组分的鉴定已通过对整个39 S亚基进行蛋白水解双链化，然后通过液相色谱和质谱分析所得肽来实现。利用肽序列信息检索人类EST数据库，拼接出完整的编码序列。人线粒体39 S亚基有48种不同的蛋白质。其中28种是大肠杆菌50 S核糖体蛋白L1、L2、L3、L4、L7/L12、L9、L10、L11、L13、L14、L15、L16、L17、L18、L19、L20、L21、L22、L23、L24、L27、L28、L30、L32、L33、L34、L35和L36的同源物。几乎所有这些蛋白质在果蝇、秀丽隐杆线虫和酿酒酵母线粒体核糖体中都有同源物。在获得的肽中或通过分析可用的数据库，没有发现原核核糖体蛋白L5、L 6、L25、L29和L31的线粒体同源物。39个S亚基中存在的其余20种蛋白质是线粒体核糖体特异性的。这组蛋白质在细菌、叶绿体、古细菌或胞质核糖体中没有明显的同源物。除了两种蛋白质外，所有蛋白质在D中都有明确的同源物。melanogaster的基因组中，而C.优雅的20个线粒体特异性39 S蛋白中有10个在S.啤酒。在拟南芥基因组中可以鉴定出这些新的核糖体蛋白中的2种的同源物。
Identification of all the protein components of the large subunit (39 S) of the mammalian mitochondrial ribosome has been achieved by carrying out proteolytic digestions of whole 39 S subunits followed by analysis of the resultant peptides by liquid chromatography and mass spectrometry. Peptide sequence information was used to search the human EST data bases and complete coding sequences were assembled. The human mitochondrial 39 S subunit has 48 distinct proteins. Twenty eight of these are homologs of the Escherichia coli 50 S ribosomal proteins L1, L2, L3, L4, L7/L12, L9, L10, L11, L13, L14, L15, L16, L17, L18, L19, L20, L21, L22, L23, L24, L27, L28, L30, L32, L33, L34, L35, and L36. Almost all of these proteins have homologs in Drosophila melanogaster, Caenorhabditis elegans, and Saccharomyces cerevisiae mitochondrial ribosomes. No mitochondrial homologs to prokaryotic ribosomal proteins L5, L6, L25, L29, and L31 could be found either in the peptides obtained or by analysis of the available data bases. The remaining 20 proteins present in the 39 S subunits are specific to mitochondrial ribosomes. Proteins in this group have no apparent homologs in bacterial, chloroplast, archaebacterial, or cytosolic ribosomes. All but two of the proteins has a clear homolog in D. melanogaster while all can be found in the genome of C. elegans. Ten of the 20 mitochondrial specific 39 S proteins have homologs in S. cerevisiae. Homologs of 2 of these new classes of ribosomal proteins could be identified in the Arabidopsis thaliana genome.