Real-Time Analysis of Molecular Conformation Using Silicon Electrophotonic Biosensors
Real-Time Analysis of Molecular Conformation Using Silicon Electrophotonic Biosensors
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DOI：
10.1021/acsphotonics.7b00580
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发表时间：
2017-09
期刊：
影响因子：
7
通讯作者：
José Juan-Colás;T. Krauss;S. Johnson
中科院分区：
文献类型：
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作者：
José Juan-Colás;T. Krauss;S. Johnson
硅微环谐振器因其高灵敏度和低成本的大规模生产前景而被广泛用作光学生物传感器。通常，他们只通过检测到的折射率变化来测量吸附的分子质量。在这里，我们提出并展示了一种硅微环生物传感器，它可以同时测量分子的厚度和密度以及电化学活性，从而能够实时量化表面固定的生物层和分子层的构象。分子构象的洞察是通过记录两个几何上不同的环形谐振器连接到单个接入波导的共振位移来获得的。谐振腔都支持单个TE偏振光模式，但具有不同的宽度(480和580 nm)；因此，它们的逝去场范围非常不同，提供了与传感器表面分子层相互作用的不同深度分辨率。通过将来自T的光学位移结合在一起。
Silicon microring resonators are widely used as optical biosensors because of their high sensitivity and promise of low-cost mass-manufacturing. Typically, they only measure the adsorbed molecular mass via the refractive index change they detect. Here, we propose and demonstrate a silicon microring biosensor that can measure molecular thickness and density as well as electrochemical activity simultaneously, thereby enabling quantification of the conformation of surface-immobilized biological and molecular layers in real time. Insight into the molecular conformation is obtained by recording the resonance shift from two geometrically distinct ring-resonators connected to a single access waveguide. The resonant cavities both support a single TE polarized optical mode but have different widths (480 and 580 nm); the extent of their evanescent fields is thus very different, providing different depth-resolution of the interaction with a molecular layer on the sensor surface. By combining the optical shift from t...