Molecular heterogeneity along the dorsal-ventral axis of the murine hippocampal CA1 field: A microarray analysis of gene expression
Molecular heterogeneity along the dorsal-ventral axis of the murine hippocampal CA1 field: A microarray analysis of gene expression
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DOI：
10.1016/j.neuroscience.2005.08.082
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Hen, R
中科院分区：
文献类型：
--
作者：
Leonardo, ED;Richardson-Jones, JW;Hen, R
人们对海马中隔颞叶、背腹侧（D-V）轴的功能异质性越来越感兴趣。尽管解剖连通性和病变研究指出了这些子区域的离散作用，但尚未系统地研究过该轴上差异基因表达的贡献。在这里，我们展示了Affymetrix微阵列筛选的结果，旨在鉴定成年小鼠海马CA1区域中沿D-V轴表现出显著差异表达的基因。我们的研究结果表明，绝大多数被监测的基因（bbb90 %）的组织表达水平在区域之间的差异小于20%，而不到0.1%的基因的表达水平在子区域之间的差异超过三倍。只有23个探针显示CA1背腹侧信号强度比大于3，其中18个探针在背侧富集，5个探针在腹侧富集。表达水平差异最大的探针代表了一系列在模式和信号传导中具有已知功能的基因，以及没有已知功能的基因。用地高辛标记的原位杂交选择性筛选证实了CA1亚区域化表达的存在，其中一些基因在D-V轴上表现出分级表达模式，而其他基因则局限于离散区域。我们的研究结果表明，在传统上公认的细胞结构定义的边界内，成年小鼠海马的D-V轴上存在基因表达差异。结合先前认识到的连通性差异和病变研究结果，我们的数据进一步证实了D-V轴上功能异质性的存在。(c) 2005 IBRO。Elsevier Ltd.出版。版权所有。
There has been increasing interest in functional heterogeneity along the septotemporal, dorsal-ventral (D-V) axis of the hippocampus. Although anatomical connectivity and lesion studies point to discrete roles for these sub-regions, the contribution of differential gene expression across this axis has not been systematically studied. Here we present findings from an Affymetrix microarray screen aimed at identifying genes in the CA1 region of the adult murine hippocampus that show significant differential expression along the D-V axis. Our results indicate that the vast majority of monitored genes (> 90%) had tissue expression levels that differed by less than 20% between regions, while less than 0.1% of genes had expression levels that varied more than three-fold by sub-region. Only 23 probes showed a CA1 dorsoventral signal intensity ratio greater than three: 18 enriched dorsally and five enriched ventrally. Probes with the greatest difference in expression levels represent a range of genes with known functions in patterning and signaling, as well as genes without known function. Selective screening with digoxigenin-labeled in situ hybridization confirms the existence of CA1 sub-regionalized expression, with some genes exhibiting a graded expression pattern across the D-V axis, and others restricted to a discrete region. Our findings demonstrate that there are gene expression differences across the D-V axis of the adult murine hippocampus within traditionally recognized cytoarchitecturally defined boundaries. Combined with the previously recognized differences in connectivity and results from lesion studies, our data further confirm the existence of functional heterogeneity along the D-V axis. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.