STUDIES ON PNEUMONIA VIRUS OF MICE (PVM) IN CELL CULTURE
STUDIES ON PNEUMONIA VIRUS OF MICE (PVM) IN CELL CULTURE
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小鼠肺炎病毒（PVM）细胞培养研究
DOI：
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发表时间：
1967
影响因子：
15.3
通讯作者：
P. Choppin
中科院分区：
文献类型：
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作者：
Donald H. Harter;P. Choppin
小鼠肺炎病毒（PVM）在幼仓鼠肾（BHK 21）细胞系中连续繁殖。获得的最大滴度为6.3 x 10（6）TCID（50）/ml，连续传代的产量变化很小。在BHK 21细胞中生长的PVM在抗原性上与从小鼠肺中获得的病毒相似，但在这些细胞中连续传代10次后，对小鼠的毒性稍低。由BHK 21细胞产生的病毒凝集小鼠红细胞，无需预先加热或其他处理。PVM在超浓缩液中的沉淀或硫酸铵沉淀导致感染性丧失，但血凝活性增加，这可能是由于病毒颗粒的破坏。在酒石酸钾密度梯度中，感染性病毒的主要部分以约1.15的密度沉降，而非感染性血凝素以约1.13的密度沉降。储备病毒制剂似乎含有大量非感染性血凝素。5-氟-2 ′-脱氧尿苷、5-溴-2 ′-脱氧尿苷和5-碘-2 ′-脱氧尿苷对PVM复制无抑制作用。感染细胞含有嗜酸性胞质内含物，其显示单链RNA的吖啶橙子染色特征。荧光抗体染色可见病毒抗原灶位于感染细胞的胞浆内。结果表明PVM是一种在细胞质中复制的RNA病毒。
Pneumonia virus of mice (PVM) has been serially propagated in a line of baby hamster kidney (BHK21) cells. A maximum titer of 6.3 x 10(6) TCID(50) per ml was obtained, and there was little variation in yield on serial passage. PVM grown in BHK21 cells was antigenically similar to virus obtained from the mouse lung, but was somewhat less virulent for the mouse after 10 serial passages in these cells. Virus produced by BHK21 cells agglutinated mouse erythrocytes without prior heating or other treatment. Sedimentation of PVM in the ultracentrifuge or precipitation by ammonium sulfate resulted in a loss in infectivity but an increase in hemagglutinating activity, presumably due to disruption of the virus particle. In a potassium tartrate density gradient, the major portion of infective virus sedimented at a density of approximately 1.15, and noninfective hemagglutinin, at a density of approximately 1.13. Stock virus preparations appear to contain a large amount of noninfective hemagglutinin. The replication of PVM was not inhibited by 5-fluoro-2'-deoxyuridine, 5-bromo-2'-deoxyuridine, or 5-iodo-2'-deoxyuridine. Infected cells contained eosinophilic cytoplasmic inclusions which showed the acridine orange staining characteristic of single-stranded RNA. Foci of viral antigen were observed in the cytoplasm of infected cells by fluorescent antibody staining. The results suggest that PVM is an RNA virus that replicates in the cytoplasm.