Ki-67 immunoreactivity in the differential diagnosis of pulmonary neuroendocrine neoplasms in specimens with extensive crush artifact
Ki-67 immunoreactivity in the differential diagnosis of pulmonary neuroendocrine neoplasms in specimens with extensive crush artifact
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DOI：
10.1309/qyv05gegkul2rtt
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发表时间：
2005-06-01
影响因子：
3.5
通讯作者：
Jessurun, J
中科院分区：
文献类型：
--
作者：
Aslan, DL;Gulbahce, HE;Jessurun, J
本研究的目的是评估免疫组化标记物在肺神经内分泌肿瘤鉴别诊断中的作用，特别强调在显示压碎伪影的区域保留免疫反应性。用Ki-67、嗜铬粒蛋白A、突触素和细胞角蛋白抗体对9例类癌（CT）和13例小细胞癌（SCC）的标本进行染色。免疫反应性在压碎区域中保存良好。Ki-67在所有SCC的压碎区域表达。反应性是弥漫性的或至少存在于25%的压碎区域中。相反，CT中的免疫反应区域从未超过10%。Ki-67、突触素、嗜铬粒蛋白A和细胞角蛋白的免疫反应性在有挤压伪影的组织中保存良好，并且可以可靠地解释。如果少于25%的细胞显示Ki-67反应性，则SCC的诊断应受到质疑。
The aim of the present study was to evaluate the usefulness of immunohistochemical markers in the differential diagnosis of pulmonary neuroendocrine tumors with particular emphasis on the preservation of immunoreactivity in areas showing crush artifacts. Specimens from 9 carcinoid tumors (CTs) and 13 small cell carcinomas (SCCs) with crush artifact were stained with antibodies to Ki-67, chromogranin A, synaptophysin, and cytokeratin. The immunoreactivity was well preserved in the crushed areas. Ki-67 was expressed in the crushed areas of all SCCs. Reactivity was diffuse or at least present in 25% of the crushed areas. In contrast, the immunoreactive areas in CTs never exceeded 10%. Immunoreactivity for Ki-67, synaptophysin, chromogranin A, and cytokeratin is well preserved in tissue with crush artifacts and can be interpreted reliably. The diagnosis of SCC should be questioned if fewer than 25% of cells show reactivity for Ki-67.