SOLUTION STRUCTURE OF THE GLUCOCORTICOID RECEPTOR DNA-BINDING DOMAIN
SOLUTION STRUCTURE OF THE GLUCOCORTICOID RECEPTOR DNA-BINDING DOMAIN
复制标题
DOI：
10.1126/science.2115209
复制
发表时间：
1990-07-13
期刊：
影响因子：
56.9
通讯作者：
KAPTEIN, R
中科院分区：
文献类型：
--
作者：
HARD, T;KELLENBACH, E;KAPTEIN, R
糖皮质激素受体的DNA结合域(DBD)的三维结构已通过核磁共振波谱和距离几何方法确定。含有两个“锌指”结构域的71个残基蛋白质片段的结构是基于一大组质子-质子距离，这些距离来自于核Overhauser增强谱，先前发现的二级结构元素中的氢键，以及保守的半胱氨酸残基对两个锌原子的配位。发现DBD由球状小体组成，手指区域从球状小体向外延伸。提出了DBD与糖皮质激素反应元件之间的二聚体复合体模型。该模型与以前的结果一致，表明DBD的特定氨基酸残基参与了蛋白质、DNA和蛋白质之间的相互作用。
The three-dimensional structure of the DNA-binding domain (DBD) of the glucocorticoid receptor has been determined by nuclear magnetic resonance spectroscopy and distance geometry. The structure of a 71-residue protein fragment containing two "zinc finger" domains is based on a large set of proton-proton distances derived from nuclear Overhauser enhancement spectra, hydrogen bonds in previously identified secondary structure elements, and coordination of two zinc atoms by conserved cysteine residues. The DBD is found to consist of a globular body from which the finger regions extend. A model of the dimeric complex between the DBD and the glucocorticoid response element is proposed. The model is consistent with previous results indicating the specific amino acid residues of the DBD are involved in protein DNA and protein-protein interactions.