Two waves of proteasome-dependent protein degradation in the hippocampus are required for recognition memory consolidation
Two waves of proteasome-dependent protein degradation in the hippocampus are required for recognition memory consolidation
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DOI：
10.1016/j.nlm.2015.02.005
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发表时间：
2015-04-01
影响因子：
2.7
通讯作者：
Schroeder, Nadja
中科院分区：
文献类型：
--
作者：
Figueiredo, Luciana S.;Dornelles, Arethuza S.;Schroeder, Nadja
健康的神经元功能和突触修饰需要蛋白质的合成和降解。越来越多的证据表明，由蛋白酶体活性介导的蛋白质更新参与了长时程突触可塑性和记忆。然而，它在记忆的不同阶段的作用仍然存在争议，以前的研究没有检查蛋白质降解在识别记忆中的可能要求。在这里，我们表明，蛋白酶体抑制剂，lactacystin（LAC），注入海马CA 1区在巩固过程中的两个特定的时间点，损害24-保留大鼠的对象识别的记忆。检索后给予LAC不影响保留。这些研究结果提供了第一个证据的蛋白酶体活性的要求，在识别记忆，表明海马中的蛋白质降解是必要的，在选择性的时间窗口的记忆巩固，并进一步了解蛋白质周转在记忆形成中的作用。(C)2015 Elsevier Inc. All rights reserved.
Healthy neuronal function and synaptic modification require a concert of synthesis and degradation of proteins. Increasing evidence indicates that protein turnover mediated by proteasome activity is involved in long-term synaptic plasticity and memory. However, its role in different phases of memory remains debated, and previous studies have not examined the possible requirement of protein degradation in recognition memory. Here, we show that the proteasome inhibitor, lactacystin (LAC), infused into the CA1 area of the hippocampus at two specific time points during consolidation, impairs 24-retention of memory for object recognition in rats. Administration of LAC after retrieval did not affect retention. These findings provide the first evidence for a requirement of proteasome activity in recognition memory, indicate that protein degradation in the hippocampus is necessary during selective time windows of memory consolidation, and further our understanding of the role of protein turnover in memory formation. (C) 2015 Elsevier Inc. All rights reserved.