Clostridium folliculivorans sp. nov., isolated from soil samples of an organic paddy in Japan.
Clostridium folliculivorans sp. nov., isolated from soil samples of an organic paddy in Japan.
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DOI：
10.1099/ijsem.0.005876
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发表时间：
2023-04
影响因子：
2.8
通讯作者：
J. Tazawa;Hisami Kobayashi;Yasuhiro Tanizawa;A. Uchino;F. Tanaka;Y. Urashima;S. Miura;M. Sakamoto-M.-Sakamot
中科院分区：
文献类型：
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作者：
J. Tazawa;Hisami Kobayashi;Yasuhiro Tanizawa;A. Uchino;F. Tanaka;Y. Urashima;S. Miura;M. Sakamoto-M.-Sakamot
从日本有机水稻土样中分离到两株革兰氏阴性、末端内孢子形成、杆状、耐氧的细菌菌株，命名为D1- 1 T和B3。菌株D1- 1 T在15-37 °C、pH 5.0-7.3和高达0.5%（w/v）NaCl下生长。16 S rRNA基因的系统发育分析表明，菌株D1- 1 T属于梭菌属，与玉米梭菌CSC 2 T（99.7%）、溶真菌梭菌TW 1 T（99.7%）和食蟹梭菌CT 4 T（99.3%）的亲缘关系最近。对D1- 1 T和B3菌株进行全基因组测序，结果表明二者无明显区别，平均核苷酸同一性值为99.7%。两个新分离株与其近缘种的平均核苷酸同源性（低于91.1%）和数字DNA-DNA杂交（低于43.6%）表明，D1- 1 T和B3菌株与其近缘种很容易区分。一种新的梭状芽孢杆菌种，食毛囊梭状芽孢杆菌新种，基于基因型和表型数据提出了模式菌株D1- 1 T（=MAFF 212477 T =DSM 113523 T）。
Two Gram-stain-negative, terminal endospore-forming, rod-shaped and aerotolerant bacterial strains designated D1-1T and B3 were isolated from soil samples of an organic paddy in Japan. Strain D1-1T grew at 15-37 °C, pH 5.0-7.3, and with up to 0.5 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene revealed that strain D1-1T belonged to the genus Clostridium and was closely related to Clostridium zeae CSC2T (99.7 % sequence similarity), Clostridium fungisolvens TW1T (99.7 %) and Clostridium manihotivorum CT4T (99.3 %). Strains D1-1T and B3 were whole-genome sequenced and indistinguishable, with an average nucleotide identity value of 99.7 %. The average nucleotide identity (below 91.1 %) and digital DNA-DNA hybridization (below 43.6 %) values between the two novel isolates and their corresponding relatives showed that strains D1-1T and B3 could be readily distinguished from their closely related species. A novel Clostridium species, Clostridium folliculivorans sp. nov., with type strain D1-1T (=MAFF 212477T=DSM 113523T), is proposed based on genotypic and phenotypic data.