Transcriptome profiling of Lilium longiflorum generative cells by cDNA microarray
Transcriptome profiling of Lilium longiflorum generative cells by cDNA microarray
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DOI：
10.1007/s00299-006-0300-9
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发表时间：
2007-07-01
影响因子：
6.2
通讯作者：
Bhalla, Prem L.
中科院分区：
文献类型：
--
作者：
Okada, Takashi;Singh, Mohan B.;Bhalla, Prem L.
由单细胞小孢子不对称分裂产生的生殖细胞，进一步进行有丝分裂产生两个参与双受精的精子细胞。对长花百合生殖细胞cDNA文库的EST分析表明，这些细胞中转录了多种基因。本研究利用斑点cDNA芯片分析了百合生殖细胞中表达基因的细胞特异性。将微阵列载玻片与来自生殖细胞和其他组织（成熟花粉、单核小孢子、子房、根尖和茎）的转录本制备的标记探针杂交。分层聚类结果显示，430个生殖细胞基因转录本中有356个（83%）在生殖细胞中表达上调。38%的生成细胞富集转录本被赋予了假定的功能，其中大量基因涉及蛋白质目的地和信号转导。这些结果表明，开花植物基因的一个子集在生殖细胞中表达受到严格控制和上调，以实现其特化功能。因此，这些数据代表了在生殖细胞中被确定为上调的基因的显著增加，并将允许对大量开花植物雄性配子表达基因进行功能分析。
The generative cell, which is produced by asymmetric division of the unicellular microspore, undergoes further mitotic division to produce two sperm cells that take part in double fertilization. Expressed sequence tag (EST) analysis of Lilium longiflorum (lily) generative cell cDNA library has shown that a diverse complement of genes is transcribed in these cells. Here we address the cell specificity of genes expressed in lily generative cell by using spotted cDNA microarray. Microarray slides were hybridized with labeled probes prepared from transcripts originating from generative cells and other tissues (mature pollen, uninucleate microspore, ovary, root tip, and shoot). The hierarchical clustering revealed that 356 of 430 gene transcripts (83%) of generative-cell genes were up regulated in generative cells. Thirty-eight percent of generative-cell-enriched transcripts were assigned their putative functions, with an abundance of genes involved in protein destination and signal transduction. These results suggest that the expression of a subset of flowering plant genes is tightly controlled and up-regulated in generative cells in order to implement their specialized function. These data thus represent a significant increase in the genes identified as being up-regulated in generative cells and would allow functional analysis of a large number of flowering plant male gamete expressed genes.